Zinedine A, Juan C, Soriano J M, Moltó J C, Idrissi L, Mañes J
Laboratory of Food Toxicology, Institut National d'Hygiène (INH), 27 Avenue Ibn Batouta, BP 769 Agdal, Rabat, Morocco.
Int J Food Microbiol. 2007 Apr 1;115(1):124-7. doi: 10.1016/j.ijfoodmicro.2006.10.013. Epub 2007 Jan 9.
Fifty eight (58) samples of cereals for human consumption (20 corn flour, 17 wheat flour) and poultry feeds (n=21) purchased from popular markets of Rabat in Morocco were analyzed for aflatoxins (AF) by HPLC with immunoaffinity column (IAC) clean-up and fluorimetric detection. The incidence of AF in corn, wheat flour and poultry feeds was about 80, 17.6 and 66.6% respectively. High contamination levels were found in corn and poultry feeds samples. Levels of contamination of analyzed samples with AFB1 ranged from 0.23 to 11.2, 0.03 to 0.15 and 0.05 to 5.38 ng/g for corn, wheat and poultry feeds respectively. Results showed also that the contamination of 10% of samples of corn was higher than the limit set by EU regulations for AFB1 and total AF. This is the first report on the determination of AF in corn, wheat and poultry feeds from Morocco by HPLC and IAC.
从摩洛哥拉巴特的大众市场购买了58份供人类食用的谷物样本（20份玉米粉、17份小麦粉）和家禽饲料样本（n = 21），采用配有免疫亲和柱（IAC）净化和荧光检测的高效液相色谱法（HPLC）对其黄曲霉毒素（AF）进行分析。玉米、小麦粉和家禽饲料中AF的发生率分别约为80%、17.6%和66.6%。在玉米和家禽饲料样本中发现了高污染水平。分析样本中黄曲霉毒素B1（AFB1）的污染水平，玉米为0.23至11.2 ng/g，小麦为0.03至0.15 ng/g，家禽饲料为0.05至5.38 ng/g。结果还表明，10%的玉米样本污染高于欧盟法规对AFB1和总AF设定的限值。这是关于采用HPLC和IAC测定摩洛哥玉米、小麦和家禽饲料中AF的首份报告。
