Bloor J H, Meisler M H, Nielsen J T
J Biol Chem. 1981 Jan 10;256(1):373-7.
Extensive genetic variation in structure and rate of synthesis of pancreatic amylase has been identified among strains of mice. The relative rate of synthesis of amylase varies from 0.15 in strain YBR to 0.26 in strain C3H. The number of electrophoretic isozymes of pancreatic amylase varies between one and four. In each strain with multiple amylase isozymes, a characteristic quantitative distribution of protein among the isozymes is observed. Isozyme proportions are determined by the relative rates of synthesis of each component. Congenic lines with different amylase phenotypes have been established. Genetic analysis reveals the close linkage of cis-acting sites determining rate of synthesis and electrophoretic mobility of mouse pancreatic amylase.
已在小鼠品系中鉴定出胰腺淀粉酶合成结构和速率的广泛遗传变异。淀粉酶的相对合成速率在YBR品系中为0.15，在C3H品系中为0.26。胰腺淀粉酶的电泳同工酶数量在1至4个之间变化。在每个具有多种淀粉酶同工酶的品系中，观察到同工酶之间蛋白质的特征性定量分布。同工酶比例由各组分的相对合成速率决定。已建立了具有不同淀粉酶表型的同类系。遗传分析揭示了决定小鼠胰腺淀粉酶合成速率和电泳迁移率的顺式作用位点紧密连锁。
