开发一种基于半定量简并实时PCR的检测方法，用于估计复杂细菌样本中丁酰辅酶A（CoA）CoA转移酶基因的数量。
Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
作者信息
Louis Petra, Flint Harry J
机构信息
Microbial Ecology Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
出版信息
Appl Environ Microbiol. 2007 Mar;73(6):2009-12. doi: 10.1128/AEM.02561-06. Epub 2007 Jan 26.
Abstract
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.
摘要
我们描述了一种新的简并实时聚合酶链反应方法，该方法基于丁酰辅酶A（CoA）CoA转移酶基因的检测，同时对系统发育上不同的产丁酸细菌进行定量。此途径存在于人类结肠中数量众多的产丁酸菌群体中，因此该检测方法可评估微生物群的产丁酸能力。
相似文献
1
Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
Appl Environ Microbiol. 2007 Mar;73(6):2009-12. doi: 10.1128/AEM.02561-06. Epub 2007 Jan 26.
2
Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene.
Environ Microbiol. 2010 Feb;12(2):304-14. doi: 10.1111/j.1462-2920.2009.02066.x. Epub 2009 Oct 5.
3
Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer.
FEMS Microbiol Lett. 2007 Apr;269(2):240-7. doi: 10.1111/j.1574-6968.2006.00629.x. Epub 2007 Jan 15.
4
Quantification of butyryl CoA:acetate CoA-transferase genes reveals different butyrate production capacity in individuals according to diet and age.
FEMS Microbiol Lett. 2011 Mar;316(2):130-5. doi: 10.1111/j.1574-6968.2010.02197.x. Epub 2011 Jan 17.
5
Function and Phylogeny of Bacterial Butyryl Coenzyme A:Acetate Transferases and Their Diversity in the Proximal Colon of Swine.
Appl Environ Microbiol. 2016 Oct 27;82(22):6788-6798. doi: 10.1128/AEM.02307-16. Print 2016 Nov 15.
6
Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer's disease.
J Microbiol. 2018 Oct;56(10):760-771. doi: 10.1007/s12275-018-8297-7. Epub 2018 Aug 22.
7
A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria.
Microbiology (Reading). 2006 Jan;152(Pt 1):179-185. doi: 10.1099/mic.0.28412-0.
8
[Genes and gene clusters involved in microbial butyrate-producing pathway of human and animal gastrointestinal tract].
Wei Sheng Wu Xue Bao. 2012 Oct 4;52(10):1181-6.
9
Consumption of partially hydrolysed guar gum stimulates Bifidobacteria and butyrate-producing bacteria in the human large intestine.
Benef Microbes. 2015;6(4):451-5. doi: 10.3920/BM2014.0118. Epub 2015 Feb 12.
10
6-Oxocyclohex-1-ene-1-carbonyl-coenzyme A hydrolases from obligately anaerobic bacteria: characterization and identification of its gene as a functional marker for aromatic compounds degrading anaerobes.
Environ Microbiol. 2008 Jun;10(6):1547-56. doi: 10.1111/j.1462-2920.2008.01570.x. Epub 2008 Feb 28.
引用本文的文献
1
Characterization of gut dysbiosis and intestinal barrier dysfunction in patients with metabolic dysfunction-associated steatotic liver disease and chronic kidney disease: a comparative study.
Sci Rep. 2025 May 3;15(1):15481. doi: 10.1038/s41598-025-00237-6.
2
Enhancing immunomodulation in cyclophosphamide-induced immunosuppressed mice through targeted modulation of butyrate-producing gut microbiota via oral administration of astragalus polysaccharides.
Food Sci Nutr. 2024 Aug 5;12(10):7683-7695. doi: 10.1002/fsn3.4386. eCollection 2024 Oct.
3
Crosstalk between butyrate oxidation in colonocyte and butyrate-producing bacteria.
iScience. 2024 Aug 31;27(9):110853. doi: 10.1016/j.isci.2024.110853. eCollection 2024 Sep 20.
4
Colorectal cancer and inulin supplementation: the good, the bad, and the unhelpful.
Gastroenterol Rep (Oxf). 2024 Jul 9;12:goae058. doi: 10.1093/gastro/goae058. eCollection 2024.
5
Group membership, not diet, structures the composition and functional potential of the gut microbiome in a wild primate.
mSphere. 2024 Jul 30;9(7):e0023324. doi: 10.1128/msphere.00233-24. Epub 2024 Jun 28.
6
Swapping White for High-Fibre Bread Increases Faecal Abundance of Short-Chain Fatty Acid-Producing Bacteria and Microbiome Diversity: A Randomized, Controlled, Decentralized Trial.
Nutrients. 2024 Mar 28;16(7):989. doi: 10.3390/nu16070989.
7
Distinct alterations of gut microbiota between viral- and non-viral-related hepatocellular carcinoma.
Appl Microbiol Biotechnol. 2024 Dec;108(1):34. doi: 10.1007/s00253-023-12845-1. Epub 2024 Jan 6.
8
Intestinal acetate and butyrate availability is associated with glucose metabolism in healthy individuals.
iScience. 2023 Nov 16;26(12):108478. doi: 10.1016/j.isci.2023.108478. eCollection 2023 Dec 15.
9
Short-Chain Fatty-Acid-Producing Micro-Organisms Regulate the Pancreatic FFA2-Akt/PI3K Signaling Pathway in a Diabetic Rat Model Affected by Pumpkin Oligosaccharides.
Foods. 2023 Sep 25;12(19):3559. doi: 10.3390/foods12193559.
10
Long-term benefit of DAAs on gut dysbiosis and microbial translocation in HCV-infected patients with and without HIV coinfection.
Sci Rep. 2023 Sep 2;13(1):14413. doi: 10.1038/s41598-023-41664-7.
本文引用的文献
1
Reduced dietary intake of carbohydrates by obese subjects results in decreased concentrations of butyrate and butyrate-producing bacteria in feces.
Appl Environ Microbiol. 2007 Feb;73(4):1073-8. doi: 10.1128/AEM.02340-06. Epub 2006 Dec 22.
2
Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale.
Appl Environ Microbiol. 2006 Sep;72(9):6371-6. doi: 10.1128/AEM.00701-06.
3
Two routes of metabolic cross-feeding between Bifidobacterium adolescentis and butyrate-producing anaerobes from the human gut.
Appl Environ Microbiol. 2006 May;72(5):3593-9. doi: 10.1128/AEM.72.5.3593-3599.2006.
4
A novel class of CoA-transferase involved in short-chain fatty acid metabolism in butyrate-producing human colonic bacteria.
Microbiology (Reading). 2006 Jan;152(Pt 1):179-185. doi: 10.1099/mic.0.28412-0.
5
Colonic microbiota signatures across five northern European countries.
Appl Environ Microbiol. 2005 Jul;71(7):4153-5. doi: 10.1128/AEM.71.7.4153-4155.2005.
6
pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon.
Appl Environ Microbiol. 2005 Jul;71(7):3692-700. doi: 10.1128/AEM.71.7.3692-3700.2005.
7
Divergence and redundancy of 16S rRNA sequences in genomes with multiple rrn operons.
J Bacteriol. 2004 May;186(9):2629-35. doi: 10.1128/JB.186.9.2629-2635.2004.
8
Restricted distribution of the butyrate kinase pathway among butyrate-producing bacteria from the human colon.
J Bacteriol. 2004 Apr;186(7):2099-106. doi: 10.1128/JB.186.7.2099-2106.2004.
9
The microbiology of butyrate formation in the human colon.
FEMS Microbiol Lett. 2002 Dec 17;217(2):133-9. doi: 10.1111/j.1574-6968.2002.tb11467.x.
10
Influence of resistant starch on the SCFA production and cell counts of butyrate-producing Eubacterium spp. in the human intestine.
J Appl Microbiol. 2002;93(1):157-62. doi: 10.1046/j.1365-2672.2002.01679.x.
Zotero 插件