开发一种基于半定量简并实时PCR的检测方法，用于估计复杂细菌样本中丁酰辅酶A（CoA）CoA转移酶基因的数量。
Development of a semiquantitative degenerate real-time pcr-based assay for estimation of numbers of butyryl-coenzyme A (CoA) CoA transferase genes in complex bacterial samples.
作者信息
Louis Petra, Flint Harry J
机构信息
Microbial Ecology Group, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
出版信息
Appl Environ Microbiol. 2007 Mar;73(6):2009-12. doi: 10.1128/AEM.02561-06. Epub 2007 Jan 26.
Abstract
We describe a new degenerate real-time PCR approach to simultaneously quantify phylogenetically different butyrate-producing bacteria based on the detection of butyryl-coenzyme A (CoA) CoA transferase genes. This pathway is present in numerically important groups of butyrate producers within the human colon, and thus this assay estimates the butyrate-producing ability of the microbiota.
摘要
我们描述了一种新的简并实时聚合酶链反应方法，该方法基于丁酰辅酶A（CoA）CoA转移酶基因的检测，同时对系统发育上不同的产丁酸细菌进行定量。此途径存在于人类结肠中数量众多的产丁酸菌群体中，因此该检测方法可评估微生物群的产丁酸能力。
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