Minami M, Kuraishi Y, Yabuuchi K, Yamazaki A, Satoh M
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
J Neurochem. 1992 Jan;58(1):390-2. doi: 10.1111/j.1471-4159.1992.tb09324.x.
The expression of interleukin-1 beta (IL-1 beta) mRNA in the cerebral cortex, hippocampus, striatum, and thalamus of rats was studied after transient forebrain ischemia. IL-1 beta mRNA was not detected in all these regions of sham-operated control rats. IL-1 beta mRNA was induced after transient forebrain ischemia and reached a detectable level in all regions examined 15 min after the start of recirculation. The induction of IL-1 beta mRNA had a few peaks, that is, peaks were observed at 30 and 240 min in the four regions examined, and another peak was observed at 90 min in the striatum. One day after the start of recirculation, IL-1 beta mRNA levels were markedly decreased, but even 7 days after that, IL-1 beta mRNA was found at very low levels in all regions examined. The amounts of c-fos and beta-actin mRNAs on the same blots were also examined. The induction of c-fos mRNA was transient and had only one peak in all regions examined, whereas the levels of beta-actin mRNA in these regions were fairly constant throughout the recirculation period. Thus, we provide the first evidence for a characteristic expression of IL-1 beta mRNA in several brain regions after transient forebrain ischemia.
在短暂性前脑缺血后，对大鼠大脑皮质、海马体、纹状体和丘脑白细胞介素-1β（IL-1β）mRNA的表达进行了研究。在假手术对照大鼠的所有这些区域均未检测到IL-1βmRNA。短暂性前脑缺血后诱导产生IL-1βmRNA，再灌注开始15分钟后在所有检测区域达到可检测水平。IL-1βmRNA的诱导有几个峰值，即在四个检测区域30分钟和240分钟时观察到峰值，在纹状体90分钟时观察到另一个峰值。再灌注开始一天后，IL-1βmRNA水平显著下降，但即使在那之后7天，在所有检测区域仍发现IL-1βmRNA处于非常低的水平。还检测了同一印迹上c-fos和β-肌动蛋白mRNA的量。c-fos mRNA的诱导是短暂的，在所有检测区域只有一个峰值，而这些区域β-肌动蛋白mRNA的水平在整个再灌注期相当恒定。因此，我们首次为短暂性前脑缺血后几个脑区IL-1βmRNA的特征性表达提供了证据。
