Kubo Hiroyoshi, Nawa Nobuaki, Lupsea Simona Angheluta
Department of Biology, Faculty of Science, Shinshu University, Matsumoto, Japan.
J Plant Res. 2007 May;120(3):445-9. doi: 10.1007/s10265-006-0067-7. Epub 2007 Feb 3.
We isolated several mutants of Arabidopsis thaliana (L.) Heynh. that accumulated less anthocyanin in the plant tissues, but had seeds with a brown color similar to the wild-type. These mutants were allelic with the anthocyaninless1 (anl1) mutant that has been mapped at 15.0 cM of chromosome 5. We performed fine mapping of the anl1 locus and determined that ANL1 is located between the nga106 marker and a marker corresponding to the MKP11 clone. About 70 genes are located between these two markers, including three UDP-glucose:flavonoid-3-O-glucosyltransferase-like genes and a glutathione transferase gene (TT19). A mutant of one of the glucosyltransferase genes (At5g17050) was unable to complement the anl1 phenotype, showing that the ANL1 gene encodes UDP-glucose:flavonoid-3-O-glucosyltransferase. ANL1 was expressed in all tissues examined, including rosette leaves, stems, flower buds and roots. ANL1 was not regulated by TTG1.
我们分离出了拟南芥（Arabidopsis thaliana (L.) Heynh.）的几个突变体，这些突变体在植物组织中积累的花青素较少，但种子颜色与野生型相似，呈棕色。这些突变体与已定位在第5号染色体15.0 cM处的无花青素1（anl1）突变体等位。我们对anl1基因座进行了精细定位，并确定ANL1位于nga106标记和对应于MKP11克隆的标记之间。这两个标记之间大约有70个基因，包括三个UDP - 葡萄糖：类黄酮 - 3 - O - 葡萄糖基转移酶样基因和一个谷胱甘肽转移酶基因（TT19）。其中一个葡萄糖基转移酶基因（At5g17050）的突变体无法互补anl1表型，表明ANL1基因编码UDP - 葡萄糖：类黄酮 - 3 - O - 葡萄糖基转移酶。ANL1在所有检测的组织中均有表达，包括莲座叶、茎、花芽和根。ANL1不受TTG1调控。
