de Abreu Corrêa Adriana, Albarnaz Jonas Dutra, Moresco Vanessa, Poli Carlos Rogério, Teixeira Adriano Luiz, Oliveira Simões Cláudia Maria, Monte Barardi Célia Regina
Laboratório de Virologia Aplicada, Departamento de Microbiologia e Parasitologia, CCB, Campus Universitario Trindade, 88040-900 Florianópolis-SC, Brazil.
Mar Environ Res. 2007 Jun;63(5):479-89. doi: 10.1016/j.marenvres.2006.12.002. Epub 2006 Dec 21.
The State of Santa Catarina produces the greatest quantity of edible mollusks in Brazil. To guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. A self-purification or "depuration" system that eliminates Salmonella enterica serovar Typhimurium contamination from oysters has been developed and evaluated. The depuration process occurred within a closed system, in which 1000 L of water was recirculated for 24 h. The water was sterilized with ultraviolet (UV) light, chlorine, or both together. Oysters (Crassostrea gigas) artificially contaminated with S. typhimurium were harvested every 6 h. Samples of oyster tissue were excised and both the presence and numbers of bacteria were determined. Combined UV light and chlorine treatments resulted in total elimination of bacteria within 12 h. Polymerase chain reaction detected bacteria in water exposed to the three treatments. This pioneering study is the first of its kind in Brazil and represents a major contribution to commercial mollusk culture in this country.
圣卡塔琳娜州是巴西食用软体动物产量最高的地区。为确保卫生质量，应对软体动物养殖进行致病微生物污染监测。现已开发并评估了一种能消除牡蛎中肠炎沙门氏菌鼠伤寒血清型污染的自净化或“净化”系统。净化过程在封闭系统中进行，1000升水循环24小时。水用紫外线（UV）、氯或两者共同进行消毒。每隔6小时采集人工感染鼠伤寒沙门氏菌的牡蛎（太平洋牡蛎）。切除牡蛎组织样本，测定细菌的存在情况和数量。紫外线和氯联合处理在12小时内可使细菌完全消除。聚合酶链反应在接受三种处理的水中检测到细菌。这项开创性研究在巴西尚属首次，对该国商业软体动物养殖具有重大贡献。
