Ishizaka S, Yoshikawa M, Tsujii T
Third Department of Internal Medicine, Nara Medical University, Japan.
Cell Immunol. 1992 Jan;139(1):239-47. doi: 10.1016/0008-8749(92)90116-7.
It is well known that transforming growth factor-beta (TGF-beta) strikingly inhibits numerous immune functions in short-term cultures. In this study we investigated the effects of TGF-beta on the immune responses of murine spleen cells in a prolonged period of culture. The addition of exogenous TGF-beta (0.1 ng/ml) inhibited the proliferation of Con A- or LPS-stimulated spleen cells, polyclonal IgM and IgG antibody production, and NK cell activity during 4 days of the initial culture and subsequently enhanced their responses on Day 10. The augmented polyclonal IgM and IgG responses in murine spleen cells induced by LPS and TGF-beta on Day 10 were suppressed by the secondary addition of TGF-beta on Day 6. These results suggest that TGF-beta acts as an immunoregulator in prolonged period responses by immunoactivators.
众所周知，转化生长因子-β（TGF-β）在短期培养中能显著抑制多种免疫功能。在本研究中，我们调查了TGF-β在延长培养期对小鼠脾细胞免疫反应的影响。添加外源性TGF-β（0.1 ng/ml）在初始培养的4天内抑制了伴刀豆球蛋白A或脂多糖刺激的脾细胞增殖、多克隆IgM和IgG抗体产生以及NK细胞活性，随后在第10天增强了它们的反应。在第10天由脂多糖和TGF-β诱导的小鼠脾细胞中增强的多克隆IgM和IgG反应，在第6天再次添加TGF-β后受到抑制。这些结果表明，TGF-β在免疫激活剂的长期反应中作为一种免疫调节剂发挥作用。
