Molvarec Attila, Rigó János, Nagy Bálint, Walentin Szilvia, Szalay János, Füst George, Karádi István, Prohászka Zoltán
Department of Obstetrics and Gynecology, Kútvölgyi Clinical Center, Semmelweis University, Budapest, Hungary.
J Reprod Immunol. 2007 Jun;74(1-2):163-9. doi: 10.1016/j.jri.2006.12.002. Epub 2007 Feb 12.
Heat shock proteins (Hsps) are primarily known to be intracellular proteins with molecular chaperone and cytoprotective functions. However, Hsp60 and Hsp70 have been found in the serum and plasma of healthy non-pregnant individuals. We aimed to compare serum Hsp70 concentrations in healthy pregnant women with those of healthy non-pregnant women and to determine factors influencing serum Hsp70 levels in normal pregnancy. One hundred and seventy six healthy pregnant women with uncomplicated pregnancies (age, 17-44 years; gestational age, 20-41 weeks) and 81 healthy, age-matched non-pregnant women (age, 22-40 years) were enrolled in this cross-sectional study. Serum Hsp70 concentrations were measured using an enzyme-linked immunosorbent assay, and were significantly lower in healthy pregnant women than in healthy non-pregnant women (median (25-75 percentile): 0.29 (0.20-0.35)ng/ml versus 1.27 (0.86-1.72)ng/ml; p<0.001). In healthy pregnant women, there was a statistically significant negative correlation between maternal age and serum Hsp70 concentration (Spearman R=-0.35; p<0.001) and a significant positive correlation between gestational age and serum Hsp70 level (Spearman R=0.35; p<0.001). The capacity of extracellular Hsp70 to elicit innate and adaptive proinflammatory immune responses might be harmful in pregnancy and lead to immune rejection of the fetal semi-allograft. We hypothesize that decreased circulating Hsp70 levels are due to unknown regulatory mechanisms aimed at maintaining immune tolerance in pregnancy. In conclusion, serum Hsp70 concentrations are decreased in normal human pregnancy; however, further studies are needed to explain the observed differences between pregnant and non-pregnant women.
热休克蛋白（Hsps）主要被认为是具有分子伴侣和细胞保护功能的细胞内蛋白。然而，在健康未孕个体的血清和血浆中发现了Hsp60和Hsp70。我们旨在比较健康孕妇与健康未孕女性血清Hsp70浓度，并确定正常妊娠中影响血清Hsp70水平的因素。176例妊娠无并发症的健康孕妇（年龄17 - 44岁；孕周20 - 41周）和81例年龄匹配的健康未孕女性（年龄22 - 40岁）纳入了这项横断面研究。采用酶联免疫吸附测定法测量血清Hsp70浓度，健康孕妇的血清Hsp70浓度显著低于健康未孕女性（中位数（第25 - 75百分位数）：0.29（0.20 - 0.35）ng/ml对1.27（0.86 - 1.72）ng/ml；p<0.001）。在健康孕妇中，母亲年龄与血清Hsp70浓度之间存在统计学显著的负相关（Spearman R = -0.35；p<0.001），孕周与血清Hsp70水平之间存在显著正相关（Spearman R = 0.35；p<0.001）。细胞外Hsp70引发先天性和适应性促炎免疫反应的能力在妊娠中可能是有害的，并导致对胎儿半同种异体移植物的免疫排斥。我们假设循环Hsp70水平降低是由于旨在维持妊娠免疫耐受的未知调节机制所致。总之，正常妊娠中血清Hsp70浓度降低；然而，需要进一步研究来解释观察到的孕妇与未孕女性之间的差异。
