Louzir H, Ternynck T, Gorgi Y, Tahar S, Ayed K, Avrameas S
Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France.
Clin Immunol Immunopathol. 1992 Feb;62(2):160-7. doi: 10.1016/0090-1229(92)90068-y.
Sera from 56 patients with hepatitis B virus-related chronic liver disease (CLD) and 30 normal individuals as controls were examined by enzyme immunoassay (EIA) for the presence of autoantibodies directed against actin, tubulin, myosin, double-stranded (ds) DNA, polymerized human albumin, thyroglobulin, and trinitrophenyl coupled to bovine serum albumin (TNP-BSA). Patients with CLD had consistently elevated levels of IgG, IgA, and IgM antibodies directed against all the panel antigens. The percentage of patients with autoantibodies of the IgA class was particularly high: respectively, 88 and 78% of the patients had strikingly high levels of anti-actin and anti-TNP-BSA IgA autoantibodies. High amounts of IgA and IgG antibodies to polymerized albumin as well as IgG to thyroglobulin were also detected. Circulating immune complexes (CIC) were isolated from patients' sera and their autoantibody activities were tested on the same antigen panel. The autoantibodies thus detected were of the same class and possessed the same activities, although at higher values than those present in the homologous sera. These results indicate that, regardless of their origin, autoantibodies are present in high amounts in the sera of these patients. Moreover, autoantibodies participating in the formation of CIC might play a pathological role.
采用酶免疫测定法（EIA）检测了56例乙型肝炎病毒相关性慢性肝病（CLD）患者及30例作为对照的正常个体血清中针对肌动蛋白、微管蛋白、肌球蛋白、双链（ds）DNA、聚合人白蛋白、甲状腺球蛋白以及与牛血清白蛋白偶联的三硝基苯（TNP - BSA）的自身抗体。CLD患者针对所有检测抗原的IgG、IgA和IgM抗体水平持续升高。IgA类自身抗体阳性患者的比例尤其高：分别有88%和78%的患者抗肌动蛋白和抗TNP - BSA IgA自身抗体水平显著升高。还检测到大量针对聚合白蛋白的IgA和IgG抗体以及针对甲状腺球蛋白的IgG抗体。从患者血清中分离出循环免疫复合物（CIC），并在同一抗原组上检测其自身抗体活性。由此检测到的自身抗体属于同一类别，且具有相同活性，尽管其值高于同源血清中的值。这些结果表明，无论自身抗体来源如何，在这些患者的血清中都大量存在。此外，参与CIC形成的自身抗体可能发挥病理作用。
