Kim S U, Cheong Y H, Seo D C, Hur J S, Heo J S, Cho J S
Department of Biological Environment, Sunchon National University, Sunchon, 540-742, Korea.
Water Sci Technol. 2007;55(1-2):105-11. doi: 10.2166/wst.2007.007.
A heavy metal resistant bacterium Bacillus spp. strain CPB4 was isolated from heavy metal contaminated soil in Korea and further characterised. The CPB4 strain showed a high capacity for uptake of heavy metal Pb (Pb > Cd > Cu > Ni > Co > Mn > Cr > Zn) both in single and in mixed heavy metal solution. Optimal conditions for heavy metal uptakes of CPB4 strain were 20-40 degrees C culture temperature, 5-7 pH and 24 h pre-culture times. TEM showed that large amounts of the electron-dense granules (heavy metal complexes) were found mainly on the cell wall and cell membrane. Furthermore, more than 90% of adsorbed heavy metals were distributed both in cell wall and in cell membrane fractions. The amount of heavy metal uptake was remarkably decreased by reducing the crude protein contents when cells were treated by alkali solutions. Therefore, this study showed one of the possible examples for useful bioremediation.
从韩国重金属污染土壤中分离出一株耐重金属芽孢杆菌属菌株CPB4，并对其进行了进一步表征。CPB4菌株在单一和混合重金属溶液中都表现出对重金属铅（铅>镉>铜>镍>钴>锰>铬>锌）的高吸收能力。CPB4菌株吸收重金属的最佳条件为培养温度20-40℃、pH值5-7和预培养时间24小时。透射电子显微镜显示，大量电子致密颗粒（重金属络合物）主要存在于细胞壁和细胞膜上。此外，超过90%的吸附重金属分布在细胞壁和细胞膜部分。当用碱溶液处理细胞时，通过降低粗蛋白含量，重金属吸收量显著降低。因此，本研究展示了一个有用的生物修复的可能实例。
