Altenbach S B, Kuo C C, Staraci L C, Pearson K W, Wainwright C, Georgescu A, Townsend J
Plant Cell Research Institute, Dublin, CA 94568.
Plant Mol Biol. 1992 Jan;18(2):235-45. doi: 10.1007/BF00034952.
We have increased the methionine content of the seed proteins of a commercial winter variety of canola by expressing a chimeric gene encoding a methionine-rich seed protein from Brazil nut in the seeds of transgenic plants. Transgenic canola seeds accumulate the heterologous methionine-rich protein at levels which range from 1.7% to 4.0% of the total seed protein and contain up to 33% more methionine. The precursor of the methionine-rich protein is processed correctly in the seeds, resulting in the appearance of the mature protein in the 2S protein fraction. The 2S methionine-rich protein accumulates in the transgenic seeds at the same time in development as the canola 11S seed proteins and disappears rapidly upon germination of the seed. The increase in methionine in the canola seed proteins should increase the value of canola meal which is used in animal feed formulations.
我们通过在转基因植物种子中表达一个编码来自巴西坚果的富含蛋氨酸种子蛋白的嵌合基因，提高了一种商业化冬油菜品种种子蛋白中的蛋氨酸含量。转基因油菜种子积累的富含异源蛋氨酸的蛋白占种子总蛋白的比例在1.7%至4.0%之间，蛋氨酸含量最多增加了33%。富含蛋氨酸蛋白的前体在种子中被正确加工，导致成熟蛋白出现在2S蛋白组分中。富含2S蛋氨酸的蛋白在转基因种子发育过程中与油菜11S种子蛋白同时积累，并在种子萌发后迅速消失。油菜籽种子蛋白中蛋氨酸含量的增加应会提高用于动物饲料配方的油菜籽粕的价值。
