Walden W C, Hentges D J
Appl Microbiol. 1975 Nov;30(5):781-5. doi: 10.1128/am.30.5.781-785.1975.
The sensitivity of three strains of anaerobic intestinal bacteria, Clostridium perfringens, Bacteroides fragilis, and Peptococcus magnus, to the differential effects of oxygen and adverse oxidation-reduction potential was measured. The multiplication of the three organisms was inhibited in the presence of oxygen whether the medium was at a negative oxidation-reduction potential (Eh of -50 mV), poised by the intermittent addition of dithiothreitol, or at a positive oxidation-reduction potential (Eh of near +500 mV). However, when these organisms were cultured in the presence of oxygen, no inhibition was observed, even when the oxidation-reduction potential was maintained at an average Eh of +325 mV by the addition of potassium ferricyanide. When the cultures were aerated, the growth patterns of the three organisms demonstrated different sensitivities to oxygen. P. magnus was found to be the most sensitive. After 2 h of aerobic incubation, no viable organisms could be detected. B. fragilis was intermediately sensitive to oxygen with no viable organisms detected after 5 h of aerobic incubation. C. perfringens was the least sensitive. Under conditions of aerobic incubation, viable organisms survived for 10 h. During the experiments with Clostridium, no spores were observed by spore staining.
对三种肠道厌氧菌——产气荚膜梭菌、脆弱拟杆菌和大消化链球菌——对氧气及不利氧化还原电位的不同影响的敏感性进行了测定。无论培养基处于负氧化还原电位（-50 mV的氧化还原电位，通过间歇性添加二硫苏糖醇来维持）还是正氧化还原电位（接近+500 mV的氧化还原电位），这三种微生物在有氧存在的情况下繁殖均受到抑制。然而，当这些微生物在有氧条件下培养时，即使通过添加铁氰化钾将氧化还原电位维持在+325 mV的平均氧化还原电位，也未观察到抑制现象。当培养物通气时，这三种微生物的生长模式显示出对氧气的不同敏感性。发现大消化链球菌最敏感。有氧培养2小时后，无法检测到存活的微生物。脆弱拟杆菌对氧气的敏感性居中，有氧培养5小时后未检测到存活的微生物。产气荚膜梭菌最不敏感。在有氧培养条件下，存活的微生物可存活10小时。在对梭菌进行实验期间，通过芽孢染色未观察到芽孢。
