米索硝唑、肿瘤坏死因子联合热疗对小鼠肿瘤微循环的影响。
Effects on tumour microcirculation in mice of misonidazole and tumour necrosis factor plus hyperthermia.
作者信息
Fujimoto S, Kobayashi K, Takahashi M, Konno C, Kokubun M, Ohta M, Shrestha R D, Kiuchi S
机构信息
First Department of Surgery, School of Medicine, Chiba University, Japan.
出版信息
Br J Cancer. 1992 Jan;65(1):33-6. doi: 10.1038/bjc.1992.6.
We examined the effects of misonidazole (MISO) and recombinant human tumour necrosis factor (rh-TNF) on tumour blood flow in mice given hyperthermic treatments. MISO (500 mg kg-1) or rh-TNF (6 x 10(4) unit kg-1) was administered intraperitoneally (i.p.) prior to hyperthermia to nude mice bearing a xenoplanted human gastric cancer and tumour blood flow was measured by a hydrogen diffusion method based on polarographic determinations. MISO plus hyperthermia produced a temperature-dependent decrease in blood flow and, at 43.5 degrees C, the flow decreased to 15-30% of control and remained low for up to 24 h. Blood flow following rh-TNF plus hyperthermia was less than that at the same temperatures following MISO plus hyperthermia, and, at 43.5 degrees C, the flow decreased to 10-20% of control and remained low for up to 48 h. Tumour growth delay was closely related to the duration of the decrease in blood flow. Thus, the profound decrease in tumour blood flow following hyperthermia plus MISO or rh-TNF and the consequential tumour regression may well be of potential clinical significance.
我们研究了米索硝唑（MISO）和重组人肿瘤坏死因子（rh-TNF）对接受热疗的小鼠肿瘤血流的影响。在对携带人胃癌异种移植瘤的裸鼠进行热疗之前，腹腔注射（i.p.）MISO（500 mg kg-1）或rh-TNF（6 x 10(4) 单位 kg-1），并通过基于极谱测定的氢扩散法测量肿瘤血流。MISO加 热疗使血流随温度降低，在43.5摄氏度时，血流降至对照的15%-30%，并在长达24小时内保持低水平。rh-TNF加 热疗后的血流低于MISO加 热疗在相同温度下的血流，在43.5摄氏度时，血流降至对照的10%-20%，并在长达48小时内保持低水平。肿瘤生长延迟与血流减少的持续时间密切相关。因此，热疗加MISO或rh-TNF后肿瘤血流的显著减少以及随之而来的肿瘤消退可能具有潜在的临床意义。
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