Brighton C T, Lorich D G, Kupcha R, Reilly T M, Jones A R, Woodbury R A
Department of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Philadelphia 19104-6081.
Clin Orthop Relat Res. 1992 Feb(275):287-99.
Bovine capillary and microvessel pericytes were grown in monolayer in standard tissue culture medium supplemented with 10% newborn calf serum at various oxygen tensions for up to ten weeks. The pericytes synthesized alkaline phosphatase and formed colonies that mineralized. Energy dispersive X-ray spectrometry revealed the presence of calcium and phosphate, showed positive staining for collagen and glycosaminoglycan, and, most importantly, demonstrated the synthesis of osteocalcin. Cell proliferation, hydroxyproline production, and alkaline phosphatase synthesis were greatest in 3% oxygen, whereas osteocalcin production was least in 3% oxygen. These findings demonstrate that the capillary or microvessel pericyte exhibits phenotypic expressions in vitro that are similar to that of in vitro bone cells, and these expressions may be somewhat oxygen dependent. It is suggested from these findings that the capillary or microvessel pericyte may be an osteoblast precursor cell.
牛毛细血管和微血管周细胞在添加10%新生牛血清的标准组织培养基中，于不同氧张力下进行单层培养，培养时间长达十周。周细胞合成碱性磷酸酶并形成矿化的集落。能量色散X射线光谱分析显示存在钙和磷，胶原蛋白和糖胺聚糖染色呈阳性，最重要的是，证明了骨钙素的合成。细胞增殖、羟脯氨酸产生和碱性磷酸酶合成在3%氧气条件下最为显著，而骨钙素产生在3%氧气条件下最少。这些发现表明，毛细血管或微血管周细胞在体外表现出与体外骨细胞相似的表型表达，并且这些表达可能在一定程度上依赖于氧气。从这些发现推测，毛细血管或微血管周细胞可能是成骨细胞前体细胞。
