Zhai Hongbo, Dika Emi, Goldovsky Marina, Maibach Howard I
Department of Dermatology, University of California, School of Medicine, San Francisco, CA 94143-0989, USA.
Skin Res Technol. 2007 May;13(2):207-10. doi: 10.1111/j.1600-0846.2007.00218.x.
BACKGROUND/PURPOSE: If the occlusion time of a closed chamber evaporimeter on the skin is too long, saturation might occur. We previously compared an open chamber and a closed chamber device on healthy volunteers. Comparable data on stripped skin with higher evaporation rates are not available. This study compares the sensitivity and correlation of open and closed chamber devices in a tape-stripping human model. The amount of tape removed SC was also quantified with a protein assay method.
Ten healthy volunteers (six male and four female; seven Caucasians and three Asian; mean age 38+/-16) were enrolled. In a randomized manner, one forearm was measured by an open chamber device and the opposite by a closed chamber device. After recording baseline measurements, 20 strippings were taken on each test site with tape disks. Transepidermal water loss (TEWL) was measured at the end of 10 and 20 tape strippings at each test site. Stratum corneum (SC) aggregates in the strips was assayed.
The mean values obtained from two devices were similar after 10 trips and 20 strips. There was no statistically significant difference. The closed chamber device showed a slightly higher (but not significant) inter-individual coefficient of variation. SC aggregates in the strips were similar and without a statistically significant difference.
The study suggests that both devices might yield similar TEWL values on stripped human skin in vivo.
背景/目的：如果密闭腔室蒸发仪在皮肤上的封闭时间过长，可能会出现饱和现象。我们之前在健康志愿者身上比较了开放腔室和密闭腔室装置。目前尚无关于具有较高蒸发率的去角质皮肤的可比数据。本研究在胶带剥离人体模型中比较了开放腔室和密闭腔室装置的敏感性和相关性。还用蛋白质测定法对去除的角质层胶带量进行了定量。
招募了10名健康志愿者（6名男性和4名女性；7名白种人和3名亚洲人；平均年龄38±16岁）。以随机方式，用开放腔室装置测量一侧前臂，另一侧用密闭腔室装置测量。记录基线测量值后，在每个测试部位用胶带盘进行20次剥离。在每个测试部位进行10次和20次胶带剥离后测量经表皮水分流失（TEWL）。对胶带中的角质层（SC）聚集体进行分析。
在进行10次和20次剥离后，两种装置获得的平均值相似。无统计学显著差异。密闭腔室装置的个体间变异系数略高（但不显著）。胶带中的SC聚集体相似，无统计学显著差异。
该研究表明，两种装置在体内去角质的人体皮肤上可能产生相似的TEWL值。
