Torres-Medina A, Wyatt R G, Mebus C A, Underdahl N R, Kapikian A Z
J Infect Dis. 1976 Jan;133(1):22-7. doi: 10.1093/infdis/133.1.22.
One- to four-day-old gnotobiotic piglets were inoculated orally with a reovirus-like agent obtained from human infants with acute gastroenteritis. Diarrhea developed in the piglets two to seven days after inoculation and was reproduced for five serial passages in one sequence and for three passages in another. Nineteen of 21 inoculated piglets developed diarrhea; reovirus-like particles were observed in intestinal contents and/or fecal samples from 17 animals with illness and from two inoculated piglets that did not develop diarrhea. One piglet, for which daily fecal samples were examined by electron microscopy, shed the largest number of virus particles at the onset of diarrhea. Immunofluorescent antibody responses to the reovirus-like agent were detected in sera from the seven inoculated animals that were tested.
将1至4日龄的无菌仔猪经口接种从患有急性胃肠炎的人类婴儿中分离出的一种呼肠孤病毒样病原体。接种后2至7天仔猪出现腹泻，在一个序列中连续传代5次，在另一个序列中传代3次后腹泻症状重现。21只接种仔猪中有19只出现腹泻；在17只患病动物和2只未出现腹泻的接种仔猪的肠道内容物和/或粪便样本中观察到呼肠孤病毒样颗粒。对一只每天通过电子显微镜检查粪便样本的仔猪进行检测，发现其在腹泻开始时排出的病毒颗粒数量最多。在7只接受检测的接种动物的血清中检测到了针对呼肠孤病毒样病原体的免疫荧光抗体反应。
