使用荧光病毒沉淀试验检测新生牛腹泻病毒、婴儿呼肠孤病毒样腹泻病毒和一种冠状病毒。
Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.
作者信息
Peterson M W, Spendlove R S, Smart R A
出版信息
J Clin Microbiol. 1976 Mar;3(3):376-7. doi: 10.1128/jcm.3.3.376-377.1976.
Abstract
Thirty-four calf and five infant fecal specimens were tested for the neonatal calf diarrhea virus (NCDV) and for the reovirus-like infantile diarrhea agent; respectively. The procedures used were the fluorescent virus precipitin test and immune electron microscopy. Fourteen of the calf stools contained detectable NCDV, and four of the five infant stools contained the reovirus-like human agent. Infectious NCDV was detected in four of the 34 calf fecal specimens when Madin-Darby bovine kidney cell cultures that had been inoculated with supernatant fluids from stool suspensions were stained with fluorescent antibody. The 20 calf stools that did not have detectable virus were examined for the bovine corona diarrhea virus. Coronavirus was found in two of these specimens.
摘要
分别对34份小牛粪便样本和5份婴儿粪便样本进行了新生小牛腹泻病毒（NCDV）以及类呼肠孤病毒婴儿腹泻病原体检测。所采用的检测方法为荧光病毒沉淀试验和免疫电子显微镜检查。14份小牛粪便样本中检测到了可检测到的NCDV，5份婴儿粪便样本中有4份含有类呼肠孤病毒人类病原体。当用荧光抗体对接种了粪便悬液上清液的马-达二氏牛肾细胞培养物进行染色时，在34份小牛粪便样本中有4份检测到了具有传染性的NCDV。对20份未检测到病毒的小牛粪便样本进行了牛冠状病毒腹泻病毒检测。在其中两份样本中发现了冠状病毒。
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Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test.使用荧光病毒沉淀试验检测新生牛腹泻病毒、婴儿呼肠孤病毒样腹泻病毒和一种冠状病毒。
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本文引用的文献
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Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests.粪便中的呼肠孤病毒样病原体：与婴儿腹泻的关联及血清学检测的发展
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