PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.
作者信息
Lohff C J, Cease K B
机构信息
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0724.
出版信息
Nucleic Acids Res. 1992 Jan 11;20(1):144. doi: 10.1093/nar/20.1.144.
Abstract
摘要
相似文献
1
PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning.使用具有3'至5'核酸外切酶活性的热稳定聚合酶进行聚合酶链反应（PCR）可产生适用于直接克隆的平端产物。
Nucleic Acids Res. 1992 Jan 11;20(1):144. doi: 10.1093/nar/20.1.144.
2
Directional enrichment of directly cloned PCR products.直接克隆的PCR产物的定向富集
Biotechniques. 2005 Jul;39(1):40, 42, 44, 46. doi: 10.2144/05391BM03.
3
Real-time monitoring of nucleic acid dephosphorylation by using molecular beacons.
Chembiochem. 2007 Sep 3;8(13):1487-90. doi: 10.1002/cbic.200700171.
4
Stability and functional effectiveness of phosphorothioate modified duplex DNA and synthetic 'mini-genes'.硫代磷酸酯修饰的双链DNA和合成“小基因”的稳定性及功能有效性
Nucleic Acids Res. 1995 Oct 25;23(20):4134-42. doi: 10.1093/nar/23.20.4134.
5
Direct cycle sequencing with delta Taq DNA polymerase.使用δTaq DNA聚合酶进行直接循环测序。
DNA Seq. 1997;7(5):285-8. doi: 10.3109/10425179709034047.
6
Improved cloning efficiency of polymerase chain reaction (PCR) products after proteinase K digestion.蛋白酶K消化后聚合酶链反应（PCR）产物的克隆效率提高。
Nucleic Acids Res. 1991 Jan 11;19(1):184. doi: 10.1093/nar/19.1.184.
7
Polymerase dependence of autosticky polymerase chain reaction.自粘性聚合酶链反应的聚合酶依赖性
Anal Biochem. 2000 Jun 15;282(1):156-8. doi: 10.1006/abio.2000.4593.
8
Comparison of the molecular influences of NO-induced lesions in DNA strands on the reactivity of polynucleotide kinases, DNA ligases and DNA polymerases.比较 NO 诱导的 DNA 链分子损伤对多核苷酸激酶、DNA 连接酶和 DNA 聚合酶反应性的影响。
J Biochem. 2010 May;147(5):697-703. doi: 10.1093/jb/mvq003. Epub 2010 Jan 22.
9
The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion.通过N端缺失可提高Taq聚合酶催化PCR的保真度。
Gene. 1992 Mar 1;112(1):29-35. doi: 10.1016/0378-1119(92)90299-5.
10
Cloning of PCR products can be inhibited by Taq polymerase carryover.PCR产物的克隆可能会受到Taq聚合酶残留的抑制。
Biotechniques. 1994 Jan;16(1):32, 37.
引用本文的文献
1
Circular PCR as an efficient and precise umbrella of methods for the generation of circular dsDNA with staggered nicks: Mechanism and types.环形PCR：一种用于生成带有交错切口的环形双链DNA的高效精确方法集：机制与类型
Biol Methods Protoc. 2024 Aug 12;9(1):bpae051. doi: 10.1093/biomethods/bpae051. eCollection 2024.
2
Efficient One-Step Fusion PCR Based on Dual-Asymmetric Primers and Two-Step Annealing.基于双不对称引物和两步退火的高效一步融合PCR
Mol Biotechnol. 2018 Feb;60(2):92-99. doi: 10.1007/s12033-017-0050-7.
3
TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression.TA-GC克隆：一种用于重组蛋白表达的PCR产物定向克隆的新型简单通用技术。
PLoS One. 2017 Nov 1;12(11):e0186568. doi: 10.1371/journal.pone.0186568. eCollection 2017.
4
Factors affecting fidelity of DNA synthesis during PCR amplification of d(C-A)n.d(G-T)n microsatellite repeats.在d（C-A）n.d（G-T）n微卫星重复序列的PCR扩增过程中影响DNA合成保真度的因素。
Nucleic Acids Res. 1996 Jun 15;24(12):2429-34. doi: 10.1093/nar/24.12.2429.
5
Expression of mouse phosphofructokinase-M gene alternative transcripts: evidence for the conserved two-promoter system.小鼠磷酸果糖激酶-M基因可变转录本的表达：保守双启动子系统的证据
Biochem J. 1994 Oct 15;303 ( Pt 2)(Pt 2):449-53. doi: 10.1042/bj3030449.
6
Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity.硫代磷酸酯引物可提高具有校对活性的DNA聚合酶对DNA序列的扩增能力。
Nucleic Acids Res. 1992 Jul 25;20(14):3551-4. doi: 10.1093/nar/20.14.3551.
7
Perils of gene mapping with microsatellite markers.使用微卫星标记进行基因图谱绘制的风险。
Am J Hum Genet. 1992 Oct;51(4):905-9.
8
The signal recognition particle (SRP) database.信号识别颗粒（SRP）数据库。
Nucleic Acids Res. 1992 May 11;20 Suppl(Suppl):2207. doi: 10.1093/nar/20.suppl.2207.
本文引用的文献
1
Molecular cloning and characterization of the HTLV-III virus associated with AIDS.与艾滋病相关的人类嗜T淋巴细胞病毒III型（HTLV-III）的分子克隆及特性分析
Nature. 1984;312(5990):166-9. doi: 10.1038/312166a0.
2
A simple method for site-directed mutagenesis using the polymerase chain reaction.一种利用聚合酶链反应进行定点诱变的简单方法。
Nucleic Acids Res. 1989 Aug 25;17(16):6545-51. doi: 10.1093/nar/17.16.6545.
3
The polymerase chain reaction colony miniprep.
Biotechniques. 1989 Jul-Aug;7(7):696-8.
4
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.原核生物和真核生物DNA聚合酶催化的新型非模板核苷酸添加反应。
Nucleic Acids Res. 1988 Oct 25;16(20):9677-86. doi: 10.1093/nar/16.20.9677.
5
Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites.高效克隆含末端限制性内切酶识别位点的PCR扩增DNA。
Nucleic Acids Res. 1990 Oct 25;18(20):6156. doi: 10.1093/nar/18.20.6156.
6
A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors.一种使用ddT尾载体直接克隆PCR产物的简单高效方法。
Nucleic Acids Res. 1991 Mar 11;19(5):1156. doi: 10.1093/nar/19.5.1156.
7
Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.T载体的构建，一种用于直接克隆未修饰PCR产物的快速通用系统。
Nucleic Acids Res. 1991 Mar 11;19(5):1154. doi: 10.1093/nar/19.5.1154.
Zotero 插件