Mao Xiangbing, Ding Xuemei, Zeng Qiufeng, Bai Shiping, Zhang Keying, Chen Daiwen, Yu Bing, He Jun, Yu Jie, Yan Hui, Luo Junqiu, Luo Yuheng, Wang Jianping
Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Chengdu 611130, People's Republic of China.
Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Key laboratory of Animal Disease-resistant Nutrition and Feed of China Ministry of Agriculture and Rural Affairs, Key laboratory of Animal Disease-resistant Nutrition of Sichuan Province, Chengdu 611130, People's Republic of China.
Poult Sci. 2021 Mar;100(3):100938. doi: 10.1016/j.psj.2020.12.035. Epub 2021 Jan 6.
This study was conducted to explore whether dietary pectic oligosaccharide (POS) supplementation could improve gut health of broiler breeders with different egg-laying rates. A 2 × 2 factorial design was used in this study. Two hundred fifty-six Arbor Acres broiler breeders (48 wk of age), including 128 average egg-laying rate and 128 low egg-laying rate (LELR) birds, were randomly fed with the diets supplemented with or without 200 mg kg of POS (n = 8). The trial lasted for 8 wk. Compared with average egg-laying rate broiler breeders, LELR broiler breeders had lower laying rate and qualified egg rate (P < 0.05), higher egg weight and feed conversion ratio (P < 0.05), higher malondialdehyde (MDA) levels in the jejunum (P < 0.05), higher IL-6 (P < 0.05) and tumor necrosis factor α (TNF-α) (P = 0.07) mRNA expressions in the jejunal mucosa, and lower microflora diversity in cecal digesta. Dietary POS supplementation increased egg weight of broiler breeders (P < 0.05), enhanced superoxide dismutase activity in the jejunum (P < 0.05), decreased MDA level in the jejunum (P < 0.05), upregulated zonula occluden 1 mRNA expression in the jejunal mucosa (P < 0.05), downregulated IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05). In addition, in LELR broiler breeders, POS administration enhanced villus height (P = 0.08) and ZO-2 mRNA expression (P = 0.09) in the jejunal mucosa, alleviated the increasing MDA level in the jejunum (P < 0.05) and IL-6 and TNF-α mRNA expressions in the jejunal mucosa (P < 0.05), and regulated relative abundance of some microbiota (including the phylum and genus, P < 0.05). These results suggest that supplementing POS in diets may elevate gut health via improvement of intestinal barrier function, antioxidant capacity, and microbiota composition in broiler breeders with different egg-laying rates.
本研究旨在探讨日粮中添加果胶寡糖（POS）是否能改善不同产蛋率的肉种鸡的肠道健康。本研究采用2×2析因设计。256只艾维茵肉种鸡（48周龄），其中128只平均产蛋率的和128只低产蛋率（LELR）的鸡，被随机分为两组，分别饲喂添加或不添加200 mg/kg POS的日粮（n = 8）。试验持续8周。与平均产蛋率的肉种鸡相比，LELR肉种鸡的产蛋率和合格种蛋率较低（P < 0.05），蛋重和饲料转化率较高（P < 0.05），空肠中丙二醛（MDA）水平较高（P < 0.05），空肠黏膜中白细胞介素6（IL-6）（P < 0.05）和肿瘤坏死因子α（TNF-α）（P = 0.07）的mRNA表达较高，盲肠内容物中的微生物多样性较低。日粮中添加POS可增加肉种鸡的蛋重（P < 0.05），提高空肠中超氧化物歧化酶活性（P < 0.05），降低空肠中MDA水平（P < 0.05），上调空肠黏膜中闭合蛋白1 mRNA表达（P < 0.05），下调空肠黏膜中IL-6和TNF-α mRNA表达（P < 0.05），并调节某些微生物群的相对丰度（包括门和属，P < 0.05）。此外，在LELR肉种鸡中，添加POS可提高空肠黏膜绒毛高度（P = 0.08）和ZO-2 mRNA表达（P = 0.09），缓解空肠中MDA水平的升高（P < 0.05）以及空肠黏膜中IL-6和TNF-α mRNA表达（P < 0.05），并调节某些微生物群的相对丰度（包括门和属，P < 0.05）。这些结果表明，在日粮中添加POS可能通过改善不同产蛋率肉种鸡的肠道屏障功能、抗氧化能力和微生物群组成来提高肠道健康水平。
