Potten C S, Kellett M, Rew D A, Roberts S A
CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital, Manchester.
Gut. 1992 Apr;33(4):524-9. doi: 10.1136/gut.33.4.524.
The distribution of DNA synthesising cells in the crypts of the epithelium in human small and large bowel after injection of bromodeoxyuridine into patients has been studied in relation to the position of the cells in the crypt using immunohistochemistry. Different sites of normal epithelium have been studied. The ileum has a shorter crypt and a very significantly smaller total cell population size. However, it has similar peak labelling index (LI) values to the colon, while the rectum has a lower peak LI value. The mean position of the label occurs at the 17th cell position in the ileum and at about the 22nd position in both the colon and rectum. The overall mean LI is significantly higher in the ileum at 17.8%, intermediate in the colon at 10.3%, and lowest in the rectum at 8.5%. There is thus an inverse relation between the likelihood of developing a tumour and the rate of cell proliferation as measured by the LI. Assuming a value of 8.6 hours for the duration of S, the data suggest that the cell cycle time in the mid crypt region is about 30 hours for the ileum and colon and about 37 hours for the rectum. Samples taken adjacent (within 1 cm) to a tumour show a general dampening of proliferative activity at all cell positions compared with samples taken more than 5 cm from a tumour. This is illustrated by the average LI, which is about 5.4% in the colon adjacent to a tumour compared with 10% distant; comparable values for the rectum are 4.6% and 8.5%. Samples taken from two patients with polyposis coli show distributions with a significant difference in skewness compared with normal colon and a general shifting of the distribution to the right, that is to higher cell positions. There is a significant increase in the mean cell position and the position of the peak LI in the polyposis coli samples.
通过免疫组织化学方法，研究了向患者注射溴脱氧尿苷后，人小肠和大肠上皮隐窝中DNA合成细胞的分布情况，并与隐窝中细胞的位置相关联。研究了正常上皮的不同部位。回肠的隐窝较短，总细胞群体大小明显较小。然而，其峰值标记指数（LI）值与结肠相似，而直肠的峰值LI值较低。标记的平均位置在回肠的第17个细胞位置，在结肠和直肠中约为第22个位置。回肠的总体平均LI显著更高，为17.8%，结肠居中，为10.3%，直肠最低，为8.5%。因此，发生肿瘤的可能性与通过LI测量的细胞增殖率之间存在反比关系。假设S期持续时间为8.6小时，数据表明，回肠和结肠隐窝中部区域的细胞周期时间约为30小时，直肠约为37小时。与距离肿瘤超过5厘米处采集的样本相比，在肿瘤附近（1厘米内）采集的样本在所有细胞位置的增殖活性普遍受到抑制。这通过平均LI得到说明，与距离肿瘤较远时的10%相比，结肠肿瘤附近的平均LI约为5.4%；直肠的可比数值分别为4.6%和8.5%。从两名患有结肠息肉病的患者采集的样本显示，与正常结肠相比，分布的偏度存在显著差异，并且分布总体向右移动，即向更高的细胞位置移动。结肠息肉病样本中的平均细胞位置和峰值LI位置显著增加。
