Chao Malin, Thongseesuksai Thaksaporn, Boonmars Thidarut, Laummaunwai Porntip
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Neglected, Zoonosis and Vector-Borne Disease Research Group, Khon Kaen University, Khon Kaen, Thailand.
J Parasit Dis. 2020 Jun;44(2):491-495. doi: 10.1007/s12639-020-01204-w. Epub 2020 Feb 17.
The present study evaluated the in vitro efficacy of miltefosine against cysts of spp. belonging to genotypes T3, T4 and T5. Each genotype was incubated with miltefosine at the concentration of 2.42, 4.84, 9.68, 19.36, 38.72 and 77.44 mM for different periods; 1, 3, 5, 7 d at 37 °C. The viability was assessed by staining with 0.4% trypan blue and culturing on NNA medium at 30 °C for 1 month. The results showed 100% eradication of cyst stage of all concentrations, but exhibited a different degree of activity against different genotypes. The MCC of 38.72 mM could kill genotype T4 and T5 after 1 d of incubation, whereas the killing of T3 needed MCC of 77.44 mM at the same incubation time. Miltefosine showed statistically highly significant difference (< 0.001) in comparison to non-treated control. Although our finding needs to confirm in animal models, this information may be the guideline for optimizing therapy or considered to combine with the other drugs for effective treatment.
本研究评估了米替福新对属于T3、T4和T5基因型的 种囊肿的体外疗效。将每种基因型与米替福新在浓度为2.42、4.84、9.68、19.36、38.72和77.44 mM的条件下孵育不同时间；在37°C下分别孵育1、3、5、7天。通过用0.4%台盼蓝染色并在30°C的NNA培养基上培养1个月来评估活力。结果显示，所有浓度均能100%根除囊肿阶段，但对不同基因型表现出不同程度的活性。38.72 mM的最低杀菌浓度在孵育1天后可杀死T4和T5基因型，而在相同孵育时间下，杀死T3基因型需要77.44 mM的最低杀菌浓度。与未处理的对照相比，米替福新显示出统计学上的高度显著差异（<0.001）。尽管我们的发现需要在动物模型中得到证实，但这些信息可能是优化治疗的指导原则，或者可考虑与其他药物联合用于有效治疗。
