Leung Kelvin Sze-Yin, Chan Kelvin, Bensoussan Alan, Munroe Mary J
Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region, PR China.
Phytochem Anal. 2007 Mar-Apr;18(2):146-50. doi: 10.1002/pca.962.
An HPLC-MS method using an atmospheric pressure chemical ionisation (APCI) source has been developed to assist in the differentiation of three ginseng species: Panax quinquefolium (American ginseng), P. ginseng (Chinese ginseng) and P. notoginseng (sanqi) species. The differentiation method relies on the identification of ginsenosides Rf and F11 and notoginsenoside R1. R1 is observed in both P. notoginseng and Chinese ginseng, whilst F1, is found exclusively in the American species. The presence of these compounds permits the definitive identification of the species to be made. The APCI ionisation source has been employed to tackle the matrix interference in analysing Chinese medicinal materials and to minimise the associated matrix effects that are commonly encountered with other ionisation modes. Moreover, the method allows direct interface to conventional HPLC systems. More importantly, chemical reference standards of ginsenosides are not required in this method. This technique provides an alternative approach to analysing high molecular weight polar compounds that typically encountered in complex matrices of Chinese medicinal materials.
已开发出一种使用大气压化学电离（APCI）源的高效液相色谱-质谱（HPLC-MS）方法，以协助区分三种人参属植物：西洋参（Panax quinquefolium）、人参（P. ginseng）和三七（P. notoginseng）。该区分方法基于人参皂苷Rf和F11以及三七皂苷R1的鉴定。三七和人参中均观察到R1，而F1仅存在于西洋参中。这些化合物的存在使得能够明确鉴定出该物种。APCI电离源已用于解决中药材分析中的基质干扰问题，并将其他电离模式常见的相关基质效应降至最低。此外，该方法允许与传统HPLC系统直接连接。更重要的是，该方法不需要人参皂苷的化学参考标准品。这项技术为分析中药材复杂基质中通常遇到的高分子量极性化合物提供了一种替代方法。
