Campanini Giulia, Percivalle Elena, Baldanti Fausto, Rovida Francesca, Bertaina Alice, Marchi Antonietta, Stronati Mauro, Gerna Giuseppe
Servizio di Virologia, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
J Clin Virol. 2007 Jun;39(2):119-24. doi: 10.1016/j.jcv.2007.03.009. Epub 2007 Apr 23.
Human respiratory syncytial virus (hRSV) detection in nasopharyngeal aspirates (NPAs) from infants with acute respiratory tract infection (ARTI) does not prove the hRSV etiology of the current ARTI episode. HRSV RNA quantification may help in affording this issue.
hRSV was detected by quantitative reverse transcription-PCR in NPAs taken upon admission to hospital and, whenever possible, at discharge and subsequent medical visits.
Prospective study, including 63 infants affected by either hRSV upper or lower ARTI.
Based on the kinetics of viral load, hRSV etiology was identified in 25 infants in whom hRSV load dropped from 2.5 x 10(6) upon admission (presence of respiratory symptoms) to 7.5 x 10(2)RNAcopies/ml NPA upon discharge (absence of symptoms) after a median time of 5 days, and in 19 infants, in whom hRSV load was determined at admission only, in association with clinical symptoms (2.4 x 10(6)copies/ml). Furthermore, low levels of hRSV RNA (<1 x 10(5)copies/ml NPA) identified 14 patients with non-hRSV ARTI. Finally, in 14 infants with hRSV coinfections or sequential infections, hRSV quantification defined the hRSV role in the current ARTI episode.
hRSV RNA quantification is critical in defining the hRSV role in respiratory infections.
在患有急性呼吸道感染（ARTI）的婴儿的鼻咽抽吸物（NPA）中检测到人类呼吸道合胞病毒（hRSV），并不能证明当前ARTI发作的病因是hRSV。hRSV RNA定量分析可能有助于解决这一问题。
通过定量逆转录聚合酶链反应（RT-PCR）检测入院时、尽可能在出院时及随后的就诊时采集的NPA中的hRSV。
前瞻性研究，纳入63例患有hRSV上呼吸道或下呼吸道ARTI的婴儿。
根据病毒载量的变化趋势，25例婴儿被确定为hRSV病因，其中hRSV载量在入院时（出现呼吸道症状）为2.5×10⁶，中位时间5天后出院时（无症状）降至7.5×10²RNA拷贝/ml NPA；另有19例婴儿仅在入院时检测到hRSV载量，并伴有临床症状（2.4×10⁶拷贝/ml）。此外，低水平的hRSV RNA（<1×10⁵拷贝/ml NPA）确定了14例非hRSV ARTI患者。最后，在14例患有hRSV合并感染或相继感染的婴儿中，hRSV定量分析确定了hRSV在当前ARTI发作中的作用。
hRSV RNA定量分析对于确定hRSV在呼吸道感染中的作用至关重要。
