Salgado Miguel, Kruze Juan, Collins Michael T
Instituto de Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Casilla 167, Valdivia, Chile.
J Vet Diagn Invest. 2007 Jan;19(1):99-102. doi: 10.1177/104063870701900117.
Fecal culture has been the primary method used to diagnose paratuberculosis in goats. It is laborious, slow, and expensive. Validation of enzyme-linked immunosorbent assays (ELISAs) on milk samples could make paratuberculosis testing more widely available for goat farmers. The aim of this study was to determine the accuracy of serum and milk ELISAs for paratuberculosis, relative to fecal culture, in Chilean dairy goats. Eight dairy goat herds were selected. Feces, blood, and milk samples were collected from all female goats >2 years old. Fecal samples were cultured using Herrold egg yolk medium with mycobactin J and antibiotics. Serum and milk samples were tested using a commercial ELISA kit for Mycobacterium avium subsp. paratuberculosis antibody detection. A total of 383 goats were tested by ELISA and fecal culture. The sensitivity of ELISA on serum and milk relative to fecal culture was 74.3% (95% CI: 59.8-88.8) and 60% (95% CI: 43.8-76.2), respectively. The corresponding values for ELISA specificity based on the percentage of non- M. avium subsp. paratuberculosis-infected goats testing ELISA-negative were 98.6% (95% CI: 96.6-100) and 99.3% (95% CI: 97.9-100) on serum and milk, respectively. Proportions of positive results for serum and fecal samples were significantly different, whereas the proportions of positive results for milk and fecal samples were not significantly different. The milk ELISA had a moderate level of agreement with fecal culture results (Kappa = 0.57). The paratuberculosis ELISA on goat milk samples may be a cost-effective, accurate alternative to fecal culture.
粪便培养一直是诊断山羊副结核病的主要方法。它费力、耗时且昂贵。对牛奶样本进行酶联免疫吸附测定（ELISA）的验证可以使山羊养殖户更广泛地进行副结核病检测。本研究的目的是确定在智利奶山羊中，相对于粪便培养，血清和牛奶ELISA检测副结核病的准确性。选择了8个奶山羊群。从所有2岁以上的母山羊中采集粪便、血液和牛奶样本。粪便样本使用含有分枝杆菌素J和抗生素的赫罗尔德蛋黄培养基进行培养。血清和牛奶样本使用商用ELISA试剂盒检测鸟分枝杆菌副结核亚种抗体。共有383只山羊接受了ELISA和粪便培养检测。相对于粪便培养，血清和牛奶ELISA的敏感性分别为74.3%（95%CI：59.8 - 88.8）和60%（95%CI：43.8 - 76.2）。基于未感染鸟分枝杆菌副结核亚种的山羊ELISA检测为阴性的百分比计算的ELISA特异性，血清和牛奶上的相应值分别为98.6%（95%CI：96.6 - 100）和99.3%（95%CI：97.9 - 100）。血清和粪便样本的阳性结果比例有显著差异，而牛奶和粪便样本的阳性结果比例没有显著差异。牛奶ELISA与粪便培养结果的一致性中等（Kappa = 0.57）。山羊奶样本的副结核病ELISA可能是一种经济高效、准确的粪便培养替代方法。
