Fitzgerald Scott D, Duncan Ann E, Tabaka Chris, Garner Michael M, Dieter April, Kiupel Matti
Diagnostic Center for Population and Animal Health, Michigan State University, Lansing 48910, USA.
J Zoo Wildl Med. 2007 Mar;38(1):150-3. doi: 10.1638/06-015.1.
This report describes the gross, histologic, and immunohistochemical features of ovarian dysgerminomas in two adult female mountain chicken frogs (Leptodactylusfallax) from the same zoological institution. One frog was found dead, and the other frog had been ill for several days with a bloated abdomen and lethargy. On necropsy, large, pale multilobulated masses replaced the left ovary in both frogs, and one frog also had numerous smaller nodules scattered throughout the coelomic viscera. Histologically, these masses were composed of sheets and cords of polyhedral discrete germ cells consistent with the diagnosis of dysgerminoma. Neoplastic cells stained positive with immunohistochemistry for Oct4, which has been reported to detect stem cells including germ cells in a variety of species, including humans. Ovarian tumors are uncommonly encountered in both reptiles and amphibians, and this report is the first report of dysgerminoma in any amphibian species.
本报告描述了来自同一动物园机构的两只成年雌性山鸡蛙（Leptodactylus fallax）卵巢无性细胞瘤的大体、组织学和免疫组化特征。一只青蛙被发现死亡，另一只青蛙患病数日，腹部肿胀且嗜睡。尸检时，两只青蛙的左卵巢均被大的、灰白色多叶状肿块取代，其中一只青蛙的体腔内脏器还散在分布着许多较小的结节。组织学上，这些肿块由多面体离散生殖细胞的片层和索状结构组成，符合无性细胞瘤的诊断。免疫组化显示肿瘤细胞Oct4染色呈阳性，据报道Oct4可在包括人类在内的多种物种中检测包括生殖细胞在内的干细胞。卵巢肿瘤在爬行动物和两栖动物中均不常见，本报告是首例关于任何两栖动物物种无性细胞瘤的报道。
