21世纪治疗性蛋白质的生物生产以及植物和植物细胞作为生产平台的作用。
Bioproduction of therapeutic proteins in the 21st century and the role of plants and plant cells as production platforms.
作者信息
Boehm Robert
机构信息
Institute of Plant Molecular Physiology and Biotechnology, Karlrobert Kreiten-Str 13, Bonn, University of Bonn, Germany.
出版信息
Ann N Y Acad Sci. 2007 Apr;1102(1):121-34. doi: 10.1196/annals.1408.009.
Abstract
In the last decade, the technique to genetically modify crop plants has gained more and more interest in terms of bioproduction of heterologous proteins. Plants have been discovered as a possible source for large amounts of cost effective recombinant protein. Main application fields are therapeutics for use in animal and human health, diagnostics, and technical enzymes. This review is focused on the recent progress in this field of molecular farming. After a comparison with hitherto established protein production systems, the advantages of plants as an alternative production system are discussed. An overview about the different host plants and possible expression strategies is given and the progress in commercialization of the techniques is highlighted. Finally, the role of plant cell cultures for the production of recombinant proteins is discussed.
摘要
在过去十年中，就异源蛋白的生物生产而言，转基因作物植物技术越来越受到关注。植物已被发现是大量具有成本效益的重组蛋白的可能来源。主要应用领域包括用于动物和人类健康的治疗药物、诊断试剂和工业酶。本综述聚焦于分子农业这一领域的最新进展。在与迄今已确立的蛋白质生产系统进行比较之后，讨论了植物作为替代生产系统的优势。给出了不同宿主植物和可能的表达策略的概述，并强调了该技术在商业化方面的进展。最后，讨论了植物细胞培养在重组蛋白生产中的作用。
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