Williamson V M, Long M, Theodoris G
Department of Nematology, University of California, Davis 95616.
Biochem Genet. 1991 Aug;29(7-8):313-23. doi: 10.1007/BF00554139.
Alcohol dehydrogenase (ADH) and the genes encoding this enzyme have been studied intensively in a broad range of organisms. Little, however, has been reported on ADH in the free-living nematode Caenorhabditis elegans. Extracts of wild-type C. elegans contain ADH activity and display a single band of activity on a native polyacrylamide gel. Reaction rate for alcohol oxidation is more rapid with higher molecular weight alcohols as substrate than with ethanol. Primary alcohols are preferred to secondary alcohols. C. elegans is sensitive to allyl alcohol, a compound that has been used to select for ADH-null mutants of several organisms. Allyl alcohol-resistant mutant strains were selected from ethylmethanesulfonate (EMS)-mutagenized nematode populations. ADH activity was measured in extracts from eight of these strains and was found to be low or nondetectable. These results form a basis for molecular and genetic characterization of ADH expression in C. elegans.
酒精脱氢酶（ADH）以及编码该酶的基因已在广泛的生物体中得到深入研究。然而，关于自由生活的线虫秀丽隐杆线虫中的ADH报道甚少。野生型秀丽隐杆线虫的提取物含有ADH活性，并且在天然聚丙烯酰胺凝胶上显示出一条活性带。以高分子量醇为底物时，酒精氧化的反应速率比以乙醇为底物时更快。伯醇比仲醇更受青睐。秀丽隐杆线虫对烯丙醇敏感，烯丙醇是一种已被用于筛选多种生物体中ADH缺失突变体的化合物。从经甲基磺酸乙酯（EMS）诱变的线虫群体中筛选出抗烯丙醇的突变菌株。对其中八个菌株的提取物进行了ADH活性测定，发现活性较低或无法检测到。这些结果为秀丽隐杆线虫中ADH表达的分子和遗传特征研究奠定了基础。
