Jacobs M, Dolferus R, Van den Bossche D
Laboratory of Plant Genetics, Free University of Brussels, Belgium.
Biochem Genet. 1988 Feb;26(1-2):105-22. doi: 10.1007/BF00555492.
Several mutants have been isolated at the Arabidopsis thaliana (L.) Heynh. alcohol dehydrogenase (ADH) gene locus using allyl alcohol selection on ethyl methanesulfonate (EMS)-mutagenized seeds. Eleven mutants were isolated in the ADH1-A electrophoretic allele, and 21 in the ADH1-S allele. These null mutants are characterized by the absence of measurable ADH activity and genetic data showed that the mutations were confined to the ADH1 gene locus of Arabidopsis. Eleven mutants in the ADH1-A background were further characterized at the protein and mRNA level. These experiments revealed striking differences in the ADH protein and mRNA content. Some of the mutants did not synthesize any mRNA or ADH-like protein, whereas some of them had a nearly normal level of ADH protein and mRNA. Others had a very low level of both protein and mRNA. ADH null mutants differed physiologically from the wild type by their higher sensitivity to anaerobic treatment in plants and significantly reduced resistance to acetaldehyde in suspension cultures.
利用烯丙醇对经甲磺酸乙酯（EMS）诱变的种子进行筛选，已在拟南芥（Arabidopsis thaliana (L.) Heynh.）乙醇脱氢酶（ADH）基因位点分离出多个突变体。在ADH1 - A电泳等位基因中分离出11个突变体，在ADH1 - S等位基因中分离出21个突变体。这些无效突变体的特征是缺乏可测量的ADH活性，遗传数据表明这些突变局限于拟南芥的ADH1基因位点。对ADH1 - A背景下的11个突变体在蛋白质和mRNA水平上进行了进一步表征。这些实验揭示了ADH蛋白和mRNA含量的显著差异。一些突变体不合成任何mRNA或ADH样蛋白，而其中一些突变体的ADH蛋白和mRNA水平几乎正常。其他突变体的蛋白和mRNA水平都非常低。ADH无效突变体在生理上与野生型不同，它们对植物厌氧处理的敏感性更高，并且在悬浮培养中对乙醛的抗性显著降低。
