Henan Key Laboratory of Microbial Engineering, Institute of Biology, Henan Academy of Sciences, Zhengzhou, China.
Curr Microbiol. 2012 Nov;65(5):595-600. doi: 10.1007/s00284-012-0187-3. Epub 2012 Aug 10.
A strain of genus Pseudomonas, LYBRD3-7(T) was isolated from long-term sulfonylurea herbicides applied wheat-field soil in Linying located in Henan province of China. This strain is a strictly aerobic and Gram-negative short rod-shaped bacterium with single flagellum. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this isolate as a member of Pseudomonas, and most closely to Pseudomonas tuomuerensis CGMCC 1.1365(T) (97.1 %) and P. alcaligenes IAM12411(T) (97.1 %). Morphological characters and chemotaxonomic data confirmed the affiliation of strain LYBRD3-7(T) to the genus Pseudomonas. The results of phylogenetic analysis, physiological and biochemical studies, and DNA-DNA hybridization allowed the differentiation of genotype and phenotype between strain LYBRD3-7(T) and the phylogenetic closest species with valid names. The name proposed for the new species is Pseudomonas linyingensis sp. nov. The type strain is LYBRD3-7(T) (=CGMCC 1.10701(T ) =LMG 25967(T)).
一株假单胞菌，LYBRD3-7(T)，从中国河南省临颍县长期使用磺酰脲类除草剂的麦田土壤中分离得到。该菌株是一种严格需氧、革兰氏阴性的短杆状细菌，具有单根鞭毛。基于 16S rRNA 基因序列分析的系统发育评估将该分离物鉴定为假单胞菌属的一个成员，与 Pseudomonas tuomuerensis CGMCC 1.1365(T)（97.1%）和 P. alcaligenes IAM12411(T)（97.1%）最为密切相关。形态特征和化学生物分类学数据证实了菌株 LYBRD3-7(T)属于假单胞菌属。系统发育分析、生理生化研究和 DNA-DNA 杂交的结果表明，菌株 LYBRD3-7(T)与具有有效名称的系统发育上最接近的种在基因型和表型上存在差异。新种的命名为临颍假单胞菌 sp. nov.。模式菌株为 LYBRD3-7(T)（=CGMCC 1.10701(T) =LMG 25967(T)）。
