来自塞勒尼绵枣儿和绒毛绵枣儿的半日花烷二萜的细胞毒性和免疫调节特性。

Cytotoxicity and immunomodulating characteristics of labdane diterpenes from Marrubium cylleneum and Marrubium velutinum.

作者信息

Karioti Anastasia, Skopeliti Margarita, Tsitsilonis Ourania, Heilmann Jörg, Skaltsa Helen

机构信息

Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimiopolis, Zografou, 157 71, Athens, Greece.

出版信息

Phytochemistry. 2007 Jun;68(11):1587-94. doi: 10.1016/j.phytochem.2007.03.027. Epub 2007 May 1.

DOI:10.1016/j.phytochem.2007.03.027

PMID:17475293

Abstract

From the aerial parts of Marrubium cylleneum, one labdane nor-diterpene has been isolated together with two labdane diterpenes, hitherto not known as natural products. The structures of the isolated compounds were established by means of NMR [(1)H-(1)H-COSY, (1)H-(13)C-HSQC, HMQC-TOCSY, HMBC, NOESY] and MS spectral analyses. Several diterpenoids from M. cylleneum and M. velutinum were tested for their cytotoxic effect against various cancer cell lines and their immunomodulating potential in human peripheral blood mononuclear cells in standard in vitro assays. Our results show a differential cytotoxicity of some compounds as well as their ability to improve selected lymphocyte functions.

摘要

从绵毛欧夏至草的地上部分分离出一种半日花烷型去甲二萜以及两种半日花烷型二萜，这两种二萜迄今尚未作为天然产物被发现。通过核磁共振波谱法[（1）H-（1）H-COSY、（1）H-（13）C-HSQC、HMQC-TOCSY、HMBC、NOESY]和质谱分析确定了分离出的化合物的结构。在标准体外试验中，对来自绵毛欧夏至草和绒毛欧夏至草的几种二萜类化合物针对多种癌细胞系的细胞毒性作用及其在人外周血单核细胞中的免疫调节潜力进行了测试。我们的结果显示了某些化合物的差异细胞毒性以及它们改善特定淋巴细胞功能的能力。