Mothana R A A, Grünert R, Lindequist U, Bednarski P J
Department of Pharmacognosy, Faculty of Pharmacy, Sana'a-University, Yemen.
Pharmazie. 2007 Apr;62(4):305-7.
The present work evaluated the anticancer activity of methanol extracts from 24 plants used in Yemeni traditional medicine. To evaluate the in vitro cytotoxic potency of the investigated extracts, an established microtiter plate assay based on cellular staining with crystal violet was used with 5 human cancer cell lines: two lung cancer (A-427 and LCLC-103H), two urinary bladder carcinoma (5637 and RT-112) and one breast cancer (MCF-7) line. The methanolic extracts of Dendrosicyos socotrana, Withanina aduensis, Withania riebeckii, Dracena cinnabari and Buxus hildebrandtii exhibited the highest toxicity on all tumor cell lines with IC50 values ranging between 0.29 and 5.54 microg/ml. The extracts of Jatropha unicostata and Punica protopunica showed a moderate potency on the most tumor cell lines.
本研究评估了也门传统医学中使用的24种植物甲醇提取物的抗癌活性。为了评估所研究提取物的体外细胞毒性效力，采用了一种基于结晶紫细胞染色的微孔板测定法，使用了5种人类癌细胞系：两种肺癌细胞系（A-427和LCLC-103H）、两种膀胱癌细胞系（5637和RT-112）以及一种乳腺癌细胞系（MCF-7）。索科特拉沙漠黄瓜、阿杜恩睡茄、里贝克睡茄、龙血树和希尔德布兰特氏黄杨的甲醇提取物对所有肿瘤细胞系均表现出最高毒性，IC50值在0.29至5.54微克/毫升之间。单脉麻风树和原石榴的提取物对大多数肿瘤细胞系显示出中等效力。
