Fritz I B, Wong K, Burdzy K
Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, Ontario, Canada.
J Cell Physiol. 1991 Nov;149(2):269-76. doi: 10.1002/jcp.1041490213.
Carnitine is bound by intact red blood cells, by red blood cell ghosts, and by glutaraldehyde-fixed human erythrocytes in a non-saturable, temperature-dependent manner. Binding of carnitine by these preparations is blocked by sulfhydryl reagents. Incubation or preincubation of red blood cell preparations with carnitine inhibits the aggregation of erythrocytes otherwise elicited by fibrinogen. Identical effects are obtained with red blood cell ghosts. In contrast, choline, even at high concentrations, is inactive in preventing the aggregation of erythrocytes. We discuss possible mechanisms by which carnitine favors the dispersion of red blood cells, and we present data indicating that sulfhydryl groups on erythrocyte membranes are required to permit these carnitine actions to be manifested.
肉碱可被完整的红细胞、红细胞血影以及经戊二醛固定的人红细胞以非饱和、温度依赖性方式结合。这些制剂对肉碱的结合可被巯基试剂阻断。红细胞制剂与肉碱一起孵育或预孵育可抑制原本由纤维蛋白原引发的红细胞聚集。红细胞血影也可产生相同效果。相比之下，即使高浓度的胆碱也无法阻止红细胞聚集。我们讨论了肉碱促进红细胞分散的可能机制，并给出数据表明红细胞膜上的巯基是这些肉碱作用得以显现所必需的。
