精索静脉曲张对大鼠睾丸间质细胞睾酮、细胞凋亡及 StARmRNA 表达的影响。
Effects of varicocele on testosterone, apoptosis and expression of StAR mRNA in rat Leydig cells.
机构信息
Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Asian J Androl. 2011 Mar;13(2):287-91. doi: 10.1038/aja.2010.111. Epub 2010 Nov 15.
The aim of this study was to explore the effects of varicocele on the morphology and function of Leydig cells in the rat testis. Forty male Sprague-Dawley rats were divided into two groups: the experimental group underwent surgery to create a left varicocele (VC), and the control group underwent a sham operation. Serum testosterone and intratesticular testosterone levels were measured using a radioimmunoassay after 4 and 8 weeks of operation. Leydig cells were studied for apoptosis and expression of steroidogenetic acute regulatory (StAR) protein mRNA levels. Serum testosterone levels declined after 4 and 8 weeks of operation but were not significant (P>0.05). However, the intratesticular testosterone levels after 8 weeks were significantly decreased compared with the control group (P<0.01). The mean apoptosis index of Leydig cells in the experimental group was significantly higher than that in the control group after 4 or 8 weeks (P<0.01). StAR mRNA levels in the Leydig cells of the experimental group were significantly lower compared to those of the control group (P<0.01). Our data show that varicocele did impair Leydig cell function by increasing apoptosis and suppressing the expression of the StAR protein.
本研究旨在探讨精索静脉曲张对大鼠睾丸间质细胞形态和功能的影响。40 只雄性 Sprague-Dawley 大鼠随机分为两组：实验组行左侧精索静脉曲张（VC）手术，对照组行假手术。术后 4 周和 8 周，采用放射免疫法检测血清和睾丸内睾酮水平。研究凋亡和类固醇生成急性调节蛋白（StAR）mRNA 水平。术后 4 周和 8 周，血清睾酮水平下降，但无统计学意义（P>0.05）。然而，与对照组相比，术后 8 周睾丸内睾酮水平显著降低（P<0.01）。实验组 Leydig 细胞的平均凋亡指数在术后 4 周或 8 周时明显高于对照组（P<0.01）。实验组 Leydig 细胞中的 StAR mRNA 水平明显低于对照组（P<0.01）。我们的数据表明，精索静脉曲张通过增加凋亡和抑制 StAR 蛋白的表达损害了 Leydig 细胞的功能。
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