Maynard Jennifer A, Myhre Ryan, Roy Benjamin
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
Curr Opin Chem Biol. 2007 Jun;11(3):306-15. doi: 10.1016/j.cbpa.2007.01.727. Epub 2007 May 17.
Over the past decade, microarrays have revolutionized the scientific world as dramatically as the internet has changed everyday life. From the initial applications of DNA microarrays to uncover gene expression patterns that are diagnostic and prognostic of cancer, understanding the interplay between immune responses and disease has been a prime application of this technology. More recent efforts have moved beyond genetic analysis to functional analysis of the molecules involved, including identification of immunodominant antigens and peptides as well as the role of post-translational glycosylation. Here, we focus on recent applications of microarray technology in understanding the detailed chemical biology of immune responses to disease in an effort to guide development of vaccines and other protective therapies.
在过去十年中，微阵列给科学界带来的变革，就如同互联网给日常生活带来的变化一样巨大。从DNA微阵列最初用于揭示癌症诊断和预后的基因表达模式开始，了解免疫反应与疾病之间的相互作用一直是这项技术的主要应用。最近的研究已从基因分析扩展到对相关分子的功能分析，包括确定免疫显性抗原和肽以及翻译后糖基化的作用。在此，我们重点关注微阵列技术在理解疾病免疫反应详细化学生物学方面的最新应用，以指导疫苗和其他保护性疗法的开发。
