Pereira L, Cassai E, Honess R W, Roizman B, Terni M, Nahmias A
Infect Immun. 1976 Jan;13(1):211-20. doi: 10.1128/iai.13.1.211-220.1976.
This paper reports on the variability of structural polypeptides of 53 strains of herpes simplex virus 1 isolated from Italy, Uganda, South Africa, and various locations in the United States. Most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numberous passages at variable multiplicities in cell culture and experimental animals. The acrylamide gel electrophoresis of polypeptides from purified virions revealed seven variable polypeptides. Virion polypeptides (VP) 7, 11, 13, 14, 15.2 and 23 were present in at least two isotypic forms characterized by fast and slow electrophoretic mobilities. VP8 could not be detected in three strains. In addition, VP13, 15.2, and 23 in some strains were either absent or comigrated with other polypeptides. A variety of tests showed that the variability in electrophoretic mobility of polypeptides was reproducible and could not be attributed to artifacts of purification, solubilization, or electrophoresis. Attempts to classify the strains on the basis of electrophoretic mobility of five or all seven variable polypeptides yielded 14 and 19 groups, respectively. The bulk of the strains (41 to 53) fell into six groups. Not all possible permutations of variable polypeptides were observed. Comparison of early and late passages of laboratory strains showed that in the few instances tested the variability could not be attributed to the propagation of the virus outside the human host. Clustering of strains on the basis of country of origin was not observed. Some clustering of isolates on the basis of site of isolation was observed, and the data do suggest that further analyses of isolates for evidence of a correlation between the site of localization on the human body and the structural polypeptides might be useful. Electrophoretic characterization of structural polypeptides has the potential of becoming a powerful tool for epidemiological studies of herpes simplex virus infections.
本文报道了从意大利、乌干达、南非以及美国不同地点分离出的53株单纯疱疹病毒1型结构多肽的变异性。大多数毒株在人类宿主外以低 multiplicity 传代次数有限；少数毒株的特征是在细胞培养和实验动物中以可变 multiplicity 传代多次。纯化病毒粒子的多肽进行丙烯酰胺凝胶电泳显示有7种可变多肽。病毒粒子多肽（VP）7、11、13、14、15.2和23至少以两种同型形式存在，其特征在于快速和缓慢的电泳迁移率。在三株毒株中未检测到VP8。此外，某些毒株中的VP13、15.2和23要么不存在，要么与其他多肽共迁移。各种测试表明，多肽电泳迁移率的变异性是可重复的，不能归因于纯化、溶解或电泳的假象。试图根据五种或全部七种可变多肽的电泳迁移率对毒株进行分类，分别产生了14组和19组。大部分毒株（41至53株）分为六组。并非观察到可变多肽的所有可能排列。实验室毒株早期和晚期传代的比较表明，在少数测试实例中，变异性不能归因于病毒在人类宿主外的传播。未观察到根据原产国对毒株进行聚类。观察到根据分离部位对分离株进行了一些聚类，数据确实表明进一步分析分离株以寻找人体定位部位与结构多肽之间相关性的证据可能是有用的。结构多肽的电泳表征有可能成为单纯疱疹病毒感染流行病学研究的有力工具。
