College of Life Science, Jilin University, Changchun 130021, People's Republic of China.
World J Microbiol Biotechnol. 2012 May;28(5):2257-65. doi: 10.1007/s11274-012-1033-2. Epub 2012 Mar 9.
To investigate bacterial communities between rhizosphere and non-rhizosphere soils of the wild medicinal plant Rumex patientia of Jilin, China, small subunit rRNAs (16S rDNA) from soil metagenome were amplified by polymerase chain reaction using primers specific to the domain bacteria and analysed by cloning and sequencing. The relative proportion of bacterial communities in rhizosphere soils was similar to non-rhizosphere soils in five phylogenetic groups (Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi and Planctomycetes). But there were differences in five other phylogenetic groups (Firmicutes, Bacteroidetes, Gemmatimonadetes, Verrucomicrobia and Unclassified bacteria). Over 97.24 % of the sequenced clones were found to be unique to rhizosphere and non-rhizosphere soils, while 2.76 % were shared by both of them. Our results indicate that there are differences in the composition and proportion of bacterial communities between rhizosphere and non-rhizosphere soils. Furthermore, the unique bacterial clones between rhizosphere and non-rhizosphere soils of the wild medicinal plant R. patientia have obvious differences.
为了研究中国吉林野生药用植物酸模叶蓼的根际和非根际土壤中的细菌群落，采用针对细菌域的引物，通过聚合酶链反应扩增土壤宏基因组中的小亚基 rRNA（16S rDNA），并通过克隆和测序进行分析。在五个系统发育群（变形菌门、放线菌门、酸杆菌门、绿弯菌门和浮霉菌门）中，根际土壤和非根际土壤的细菌群落相对比例相似。但是，在另外五个系统发育群（厚壁菌门、拟杆菌门、芽单胞菌门、疣微菌门和未分类细菌）中存在差异。超过 97.24%的测序克隆被发现是根际和非根际土壤所特有的，而 2.76%的克隆是两者共有的。我们的结果表明，根际和非根际土壤中的细菌群落组成和比例存在差异。此外，野生药用植物 R. patientia 的根际和非根际土壤之间的特有细菌克隆具有明显的差异。
