Department of Life Science, University of Seoul, Seoul 02504, Korea.
Univera Co., Ltd., Seoul 04782, Korea.
Molecules. 2021 Apr 30;26(9):2626. doi: 10.3390/molecules26092626.
Skin moisturization is very crucial for maintaining the flexibility, viscoelasticity, and differentiation of the epidermis and its deprivation causes several diseases from dry skin to dermatitis. , a miracle plant having diverse medicinal properties including skin moisturization effects. This study investigated for the first time the molecular mechanism targeting skin moisturization effects of the flower and its major active constituent. By treating human epidermal keratinocytes (HaCaT cells) with flower water extract (AFWE), we found that AFWE upregulated epidermal involucrin by activating the expression of protein kinase C, p38, and ERK 1/2. Additionally, it modulated filaggrin, increased aquaporin expression, and hyaluronan synthesis via a balanced regulation of HAS1 and HYAL1 protein. Similarly, it was able to protect UVB-induced photodamage. Western blot analysis, ELISA, and qRT- PCR were performed to evaluate various epidermal differentiation markers and moisturization-related factors on human epidermal keratinocytes (HaCaT cells). TLC and HPLC were used to detect and analyze the chemical constituents. Among them, we found that an active component of flower, isoorientin (IO) has a high binding affinity to all of its targeted proteins such as involucrin, PKC, P38, etc. through molecular docking assay. This study indicated that the flower and its active constituent, IO can be used as a prominent ingredient to enhance skin barrier function and improve its related pathologies.
皮肤保湿对于维持表皮的柔韧性、粘弹性和分化非常关键，其缺失会导致多种疾病，从干性皮肤到皮炎。 ，一种具有多种药用特性的神奇植物，包括保湿作用。本研究首次探讨了 花及其主要活性成分针对皮肤保湿作用的分子机制。通过用 花水提取物（AFWE）处理人表皮角质形成细胞（HaCaT 细胞），我们发现 AFWE 通过激活蛋白激酶 C、p38 和 ERK1/2 的表达来上调表皮内披蛋白。此外，它通过平衡调节 HAS1 和 HYAL1 蛋白来调节丝聚蛋白，增加水通道蛋白的表达和透明质酸的合成。同样，它能够保护 UVB 诱导的光损伤。通过 Western blot 分析、ELISA 和 qRT-PCR 评估了各种表皮分化标志物和保湿相关因子在人表皮角质形成细胞（HaCaT 细胞）上的作用。TLC 和 HPLC 用于检测和分析化学成分。其中，我们发现 花的一种活性成分异荭草苷（IO）通过分子对接实验对所有靶向蛋白（如内披蛋白、PKC、P38 等）具有很高的结合亲和力。本研究表明， 花及其活性成分 IO 可作为增强皮肤屏障功能和改善相关病理的突出成分。
