用于葡萄球菌临床分离株种水平鉴定的简化且可靠的方案。
Simplified and reliable scheme for species-level identification of Staphylococcus clinical isolates.
作者信息
Iorio Natalia Lopes Pontes, Ferreira Rosana Barreto Rocha, Schuenck Ricardo Pinto, Malvar Karoline Lourenco, Brilhante Anike Pereira, Nunes Ana Paula Ferreira, Bastos Carla Callegário Reis, Dos Santos Kátia Regina Netto
机构信息
Institute of Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
J Clin Microbiol. 2007 Aug;45(8):2564-9. doi: 10.1128/JCM.00679-07. Epub 2007 May 30.
Reliable and rapid identification of staphylococcal strains continues to be a problem faced by many microbiology laboratories. This study evaluates a simplified method that uses a flowchart to assist in the identification of 12 clinical species of Staphylococcus, including eight subspecies. A total of 198 isolates and 11 control strains were identified by the reference method, which employed 22 tests. The results were compared with those obtained by two other methods: an automated system (MicroScan WalkAway) and a simplified method composed of nine tests. The simplified scheme showed an accuracy of 98.5%, while the automated method showed an accuracy of 79.3% (P < 0.001), in identifying staphylococcal species. Atypical phenotypic profiles were detected by both the reference (55.6%) and the simplified (19.7%) methods. The simplified method proposed here was shown to be reliable, with the advantage of being more practical and economic than the reference method.
可靠且快速地鉴定葡萄球菌菌株仍是许多微生物实验室面临的一个问题。本研究评估了一种简化方法，该方法使用流程图来辅助鉴定12种葡萄球菌临床菌种，包括8个亚种。采用包含22项检测的参考方法对总共198株分离菌和11株对照菌株进行了鉴定。将结果与通过另外两种方法获得的结果进行了比较：一种自动化系统（MicroScan WalkAway）和一种由9项检测组成的简化方法。在鉴定葡萄球菌菌种方面，简化方案的准确率为98.5%，而自动化方法的准确率为79.3%（P<0.001）。参考方法（55.6%）和简化方法（19.7%）均检测到了非典型表型特征。此处提出的简化方法被证明是可靠的，具有比参考方法更实用、更经济的优点。
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