Buyse Johan, Swennen Quirine, Niewold Theo A, Klasing Kirk C, Janssens Geert P J, Baumgartner Max, Goddeeris Bruno M
Laboratory of Livestock Physiology, Immunology and Genetics, Department of Biosystems, Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium.
Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):154-9. doi: 10.1016/j.vetimm.2007.04.014. Epub 2007 May 3.
The objective of the present study was to evaluate the potential effects of dietary L-carnitine supplementation on acute phase protein response upon a lipopolysaccharide (LPS) challenge of male broiler chickens receiving a commercial broiler diet supplemented with 15 or 100 mg L-carnitine/kg or an unsupplemented (control) diet from 14 days of age onwards. At 28 days of age, eight chickens per dietary treatment were weighed and subcutaneously injected with 300 microg LPS from E. coli (100 microg LPS/ml saline) or 3 ml saline (unsupplemented group only). During the next 10 days, blood samples were taken repeatedly and analysed for their hemopexin (HX) and alpha-1 acid glycoprotein (AGP) levels. Extra dietary L-carnitine did not affect broiler performance. At day 1 postinjection, plasma HX and AGP levels were significantly increased in all treatment groups. However, the elevations in circulating HX and AGP levels were more pronounced in the L-carnitine supplemented chickens, especially in the 100mg L-carnitine group. It is concluded that extra L-carnitine in the diet of broiler chickens enhances or advances the acute phase protein response. The exact mode of action needs to be elucidated but seems to be consistent with a glucocorticoid mimicking effect.
本研究的目的是评估日粮添加L-肉碱对14日龄起接受添加15或100mg L-肉碱/kg的商品肉鸡日粮或未添加（对照）日粮的雄性肉鸡在脂多糖（LPS）刺激后急性期蛋白反应的潜在影响。28日龄时，对每个日粮处理组的8只鸡称重，并皮下注射来自大肠杆菌的300μg LPS（100μg LPS/ml生理盐水）或3ml生理盐水（仅未添加组）。在接下来的10天里，反复采集血样并分析其血红素结合蛋白（HX）和α-1酸性糖蛋白（AGP）水平。额外添加日粮L-肉碱不影响肉鸡性能。注射后第1天，所有处理组的血浆HX和AGP水平均显著升高。然而，添加L-肉碱的鸡中循环HX和AGP水平的升高更为明显，尤其是在100mg L-肉碱组。得出的结论是，肉鸡日粮中额外添加L-肉碱可增强或提前急性期蛋白反应。确切的作用方式有待阐明，但似乎与糖皮质激素模拟效应一致。
