Lin Wen-Hsin, Yu Bi, Jang Sheng-Hon, Tsen Hau-Yang
School of Pharmacy, China Medical University, No. 91, Hsueh Shih Rd., Taichung City 404, Taiwan, ROC.
Anaerobe. 2007 Jun-Aug;13(3-4):107-13. doi: 10.1016/j.anaerobe.2007.04.006. Epub 2007 Apr 25.
Systematic procedures were used to evaluate the probiotic properties of Lactobacillus fermentum (L. fermentum) strains isolated from swine and poultry. The major properties included their capabilities to adhere to the intestinal epithelium of swine and poultry, the inhibition on pathogenic bacteria, and their tolerance to the gastric juice and bile salts. Results showed that L. fermentum strains from poultry digestive tract showed better adherence to the swine intestine and chicken crop epithelial cells as compared to those strains from the swine origin. In addition, six strains from poultry and one strain from swine showed adhesion specificity to their own intestinal epithelium. Four poultry isolates and one swine isolate were able to adhere to the epithelial cells from both swine and chicken. For gastric juice and bile tolerance, most of the strains isolated from swine or poultry were acid tolerant but less strains were bile intolerant. The spent culture supernatant (SCS) of these L. fermentum strains showed antagonistic effect against the indicator bacteria, such as Escherichia coli, Salmonella spp., Shigella sonnei and some enterotoxigenic Staphylococcus aureus. From the above studies, some L. fermentum strains isolated from poultry were found to have the probiotic properties required for use in animal feed supplement. This study suggested that poultry digestive tract may serve as potential source for the isolation of probiotic lactic acid bacteria.
采用系统程序评估从猪和家禽中分离出的发酵乳杆菌菌株的益生菌特性。主要特性包括它们粘附于猪和家禽肠道上皮的能力、对病原菌的抑制作用以及对胃液和胆汁盐的耐受性。结果表明，与来自猪源的菌株相比，来自家禽消化道的发酵乳杆菌菌株对猪肠道和鸡嗉囊上皮细胞的粘附性更好。此外，来自家禽的6个菌株和来自猪的1个菌株对其自身肠道上皮表现出粘附特异性。4个家禽分离株和1个猪分离株能够粘附于猪和鸡的上皮细胞。对于胃液和胆汁耐受性，从猪或家禽中分离出的大多数菌株耐酸，但不耐胆汁的菌株较少。这些发酵乳杆菌菌株的培养上清液（SCS）对指示菌，如大肠杆菌、沙门氏菌属、宋内志贺氏菌和一些产肠毒素金黄色葡萄球菌具有拮抗作用。从上述研究中发现，一些从家禽中分离出的发酵乳杆菌菌株具有用作动物饲料添加剂所需的益生菌特性。本研究表明，家禽消化道可能是分离益生菌乳酸菌的潜在来源。
