Quinsac A, Ribaillier D, Elfakir C, Lafosse M, Dreux M
Centre Technique Interprofessionnel des Oléagineux Métropolitains, Ardon, France.
J Assoc Off Anal Chem. 1991 Nov-Dec;74(6):932-9.
Liquid chromatographic (LC) analysis of desulfated derivatives of rapeseed glucosinolates has been carried out under isocratic elution conditions with different CN-bonded stationary phases. The effects of the eluant composition (water, acetonitrile, and methanol) with the stationary phase (Zorbax CN, Lichrospher CN, and Ultrasphere CN) and temperature (20 and 50 degrees C) are described. An isocratic LC method performed at room temperature using a Lichrospher CN column and water as mobile phase is proposed. The chromatographic analysis can be done in less than 12 min, and it is easier and less expensive than the traditional gradient mode. Four commercial samples of rapeseed containing various quantities of other cruciferous seeds (wild mustard and stinkweed) as an admixture have been analyzed to determine the total glucosinolate content. Relative standard deviations of repeatability of the isocratic and gradient LC methods ranged from 0.4 to 1.7% and from 2.7 to 4.7%, respectively. Comparison of the results showed good agreement between the 2 methods (beter than 98%).
在等度洗脱条件下，使用不同的氰基键合固定相，对油菜籽硫代葡萄糖苷的脱硫酸化衍生物进行了液相色谱（LC）分析。描述了洗脱液组成（水、乙腈和甲醇）与固定相（Zorbax CN、Lichrospher CN和Ultrasphere CN）以及温度（20和50摄氏度）的影响。提出了一种在室温下使用Lichrospher CN柱并以水为流动相的等度LC方法。色谱分析可在不到12分钟内完成，且比传统梯度模式更简便、成本更低。对四个含有不同数量其他十字花科种子（野芥菜和臭草）作为掺杂物的油菜籽商业样品进行了分析，以确定总硫代葡萄糖苷含量。等度和梯度LC方法重复性的相对标准偏差分别为0.4%至1.7%和2.7%至4.7%。结果比较表明两种方法之间具有良好的一致性（优于98%）。
