在高浓度蛋白质溶液中，使用离子液体实现可逆的折叠-展开、聚集保护及多年稳定性。
Reversible folding-unfolding, aggregation protection, and multi-year stabilization, in high concentration protein solutions, using ionic liquids.
作者信息
Byrne Nolene, Wang Li-Min, Belieres Jean-Philippe, Angell C Austen
机构信息
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.
出版信息
Chem Commun (Camb). 2007 Jul 14(26):2714-6. doi: 10.1039/b618943a. Epub 2007 Apr 19.
DOI:10.1039/b618943a
PMID:17594030
Abstract
We report the reversible thermal unfolding/refolding, and long period stabilization against aggregation and hydrolysis, of >200 mg ml(-1) solutions of lysozyme in ionic liquid-rich, ice-avoiding, solvents.
摘要
我们报道了溶菌酶在富含离子液体、避免结冰的溶剂中形成的浓度大于200 mg ml⁻¹溶液的可逆热展开/重折叠，以及针对聚集和水解的长期稳定性。
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