Wang S, Chiu J F, Klyszejko-Stefanowicz L, Fujitani H, Hnilica L S
J Biol Chem. 1976 Mar 10;251(5):1471-5.
A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (Km = 6.7 X 10(-9) M) and decrease with rising salt concentration. Complexes of the NP protein fraction with homologous DNA are immunologically tissue-specific. As determined by microcomplement fixation, the NP proteins in Novikoff hepatoma are associated with the transcriptionally active, diffuse fraction of chromatin.
从大鼠肝脏和诺维科夫肝癌中分离出了一组对DNA具有亲和力的染色体非组蛋白（NP）。该组分占染色质蛋白质总量的不到5%，它优先与分级分离的同源DNA的独特双链序列结合。在低离子强度下（Km = 6.7×10⁻⁹ M），这种相互作用很强，并且会随着盐浓度的升高而减弱。NP蛋白组分与同源DNA的复合物具有免疫组织特异性。通过微量补体结合测定，诺维科夫肝癌中的NP蛋白与染色质转录活性的弥散部分相关。
