Urakami Hiroshi, Ikarashi Kumiko, Okamoto Ko, Abe Yukari, Ikarashi Tamami, Kono Takeshi, Konagaya Yukifumi, Tanaka Nobumasa
College of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences (NUPALS), 265-1 Higashijima, Niigata, Niigata 956-8603, Japan.
Appl Environ Microbiol. 2007 Sep;73(17):5679-82. doi: 10.1128/AEM.00083-07. Epub 2007 Jul 6.
The sensitivity to free chlorine of feline calicivirus (FCV), a norovirus surrogate, was examined relative to chlorine demand. When a crude suspension of FCV was treated with a sodium hypochlorite solution containing 10 microg/ml free chlorine, the extent of the decrease of viral infectivity clearly depended on the volume of the reaction mixture. The apparent sensitivity of FCV to free chlorine increased with the reduction of host cell debris, indicating that chlorine demand must be minimized to know the true sensitivity of the virus. We therefore partially purified the viruses from the host cell components and found that the infectivity of FCV was reduced by more than log 4.6 by 5 min of treatment with 300 ng/ml free chlorine.
相对于氯需求量，对猫杯状病毒（FCV，一种诺如病毒替代物）对游离氯的敏感性进行了检测。当用含有10微克/毫升游离氯的次氯酸钠溶液处理FCV的粗悬液时，病毒感染力下降的程度明显取决于反应混合物的体积。FCV对游离氯的表观敏感性随着宿主细胞碎片的减少而增加，这表明必须将氯需求量降至最低才能了解病毒的真正敏感性。因此，我们从宿主细胞成分中对病毒进行了部分纯化，发现用300纳克/毫升游离氯处理5分钟后，FCV的感染力降低了超过4.6个对数。
