Fukunaga M, Sohnaka M, Takahashi Y, Nakao M, Miyamoto K
Faculty of Pharmacy and Pharmaceutical Sciences, University of Fukuyama, Hiroshima, Japan.
J Clin Microbiol. 1993 May;31(5):1388-91. doi: 10.1128/jcm.31.5.1388-1391.1993.
Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis. The protein profiles of the borrelial isolates were variable and markedly different from that of the type strain Borrelia burgdorferi B31. The 41-kDa flagellin protein was present in all isolates, but the outer surface protein A (OspA) was absent in four isolates (HT15, HT2, HT20, and HT32). The molecular weights of the OspA proteins in six isolates were found to differ from one isolate to another. No two isolates examined had the same plasmid profile. These findings show the antigenic and genetic heterogeneity of the Japanese isolates, and some isolates are strikingly different from North American, European, and Asian strains.
从日本北海道全沟硬蜱成虫中分离出10株具有代表性的螺旋体菌株（HT2、HT7、HT10、HT15、HT17、HT19、HT20、HT22、HT32和HT59），并通过聚丙烯酰胺凝胶电泳、使用单克隆和多克隆抗体以及脉冲场凝胶电泳进行分析。疏螺旋体分离株的蛋白质谱各不相同，与模式菌株伯氏疏螺旋体B31明显不同。所有分离株中均存在41 kDa鞭毛蛋白，但4株分离株（HT15、HT2、HT20和HT32）中不存在外表面蛋白A（OspA）。发现6株分离株中OspA蛋白的分子量各不相同。所检测的分离株中没有两株具有相同的质粒图谱。这些发现表明日本分离株具有抗原性和遗传异质性，并且一些分离株与北美、欧洲和亚洲菌株显著不同。
