Catauro Michelina, Raucci Mariagrazia, Ausanio Giovanni
Department of Aerospace and Mechanical Engineering, Second University of Naples, Via Roma no. 21, Aversa, CE, Italy.
J Mater Sci Mater Med. 2008 Feb;19(2):531-40. doi: 10.1007/s10856-007-3065-y. Epub 2007 Jul 10.
Poly(epsilon-caprolactone) (PCL 6, 12 and 24 wt%) and zirconium (ZrO(2)) organic-inorganic hybrid materials have been synthesized by the sol-gel method from a multicomponent solution containing zirconium propoxide [Zr(OC(2)H(7))(4)], poly(epsilon-caprolactone) (PCL), water, chloroform (CHCl(3)). Sodium ampicillin was incorporate in the hybrid materials to verify the effect as local controlled drug delivery system. The structure of interpenetrating network is realized by hydrogen bonds between Zr-OH group (H donator) in the sol-gel intermediate species and carboxylic group (H-acceptor) in the repeating units of the polymer. The presence of hydrogen bonds between organic/inorganic components of the hybrid material was proved by FTIR analysis. The morphology of the hybrid material was studied by scanning electron microscope (SEM). The structure of a molecular level dispersion has been disclosed by atomic force microscope (AFM), pore size distribution and surface measurements. The bioactivity of the synthesized hybrid material has been showed by the formation of a layer of hydroxyapatite on the surface of PCL/ZrO(2) samples soaked in a fluid simulating the composition of the human blood plasma. Release kinetics in a simulate body fluid (SBF) have been subsequently investigated. The amount of sodium ampicillin released has been detected by UV-VIS spectroscopy and SEM. The release kinetics seems to occur in more than one stage. HPLC analysis has also been taken to ensure the integrity of ampicillin after the synthetic treatment.
通过溶胶 - 凝胶法，由包含丙醇锆[Zr(OC₂H₇)₄]、聚（ε - 己内酯）（PCL）、水、氯仿（CHCl₃）的多组分溶液合成了聚（ε - 己内酯）（PCL质量分数为6%、12%和24%）与锆（ZrO₂）的有机 - 无机杂化材料。将氨苄西林钠掺入杂化材料中，以验证其作为局部控释给药系统的效果。互穿网络结构是通过溶胶 - 凝胶中间物种中的Zr - OH基团（氢供体）与聚合物重复单元中的羧基（氢受体）之间的氢键实现的。通过傅里叶变换红外光谱（FTIR）分析证明了杂化材料有机/无机组分之间存在氢键。通过扫描电子显微镜（SEM）研究了杂化材料的形态。通过原子力显微镜（AFM）、孔径分布和表面测量揭示了分子水平分散的结构。通过在模拟人血浆成分的流体中浸泡的PCL/ZrO₂样品表面形成一层羟基磷灰石，证明了合成杂化材料的生物活性。随后研究了在模拟体液（SBF）中的释放动力学。通过紫外 - 可见光谱（UV - VIS）和SEM检测了氨苄西林钠的释放量。释放动力学似乎分多个阶段发生。还进行了高效液相色谱（HPLC）分析，以确保合成处理后氨苄西林的完整性。
