BK病毒及BK病毒DNA的传染性、致癌性和转化能力。
Infectivity, oncogenicity and transforming ability of BK virus and BK virus DNA.
作者信息
van der Noordaa J
出版信息
J Gen Virol. 1976 Mar;30(3):371-3. doi: 10.1099/0022-1317-30-3-371.
PMID:176315
Abstract
The human papovavirus, BK, appeared weakly oncogenic in newborn hamsters and was able to induce in vitro transformation of rat kidney cells. The infectivity of BK virus DNA was determined by employing the DEAE-dextran method. In human embryonic cells the infectivity was approx. 10(5) p.f.u./mug of DNA. The transforming ability of BK virus in primary rat kidney cells was measured by employing the calcium method and appeared to vary from 1 to 10 foci per mug of DNA.
摘要
人乳头多瘤空泡病毒BK在新生仓鼠中表现出弱致癌性，并且能够在体外诱导大鼠肾细胞发生转化。通过采用二乙氨基乙基葡聚糖法测定BK病毒DNA的感染性。在人胚胎细胞中，感染性约为每微克DNA 10⁵个空斑形成单位。通过采用钙法测定BK病毒在原代大鼠肾细胞中的转化能力，结果显示每微克DNA形成的转化灶数量在1至10个之间变化。
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