Drillich M, Voigt D, Forderung D, Heuwieser W
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Free University of Berlin, 14163, Germany.
J Dairy Sci. 2007 Aug;90(8):3758-63. doi: 10.3168/jds.2007-0052.
The objective of this field trial was to evaluate effects of a single administration of 2.2 mg/kg of body weight (BW) of flunixin meglumine (FM) in addition to a systemic antibiotic treatment in cows with acute puerperal metritis (APM). Outcome variables tested were proportion of cows with a fever, prevalence of chronic endometritis 18 to 22 and 32 to 35 d in milk (DIM), and reproductive performance measures in the current lactation. In addition, serum concentrations of haptoglobin and fibrinogen were analyzed. Daily milk yield within 6 d after the first treatment was recorded. Cows were examined 4 to 5 DIM by rectal palpation and vaginoscopy, and rectal temperature was measured. Fetid vulvar discharge and a body temperature > or = 39.5 degrees C were signs of APM. Cows with APM were treated in the reference group with 1.0 mg/kg of BW of ceftiofur on 3 to 5 consecutive days (CEF, n = 119). In the study group, cows received the same antibiotic treatment as in CEF and 2.2 mg/kg of BW of FM on treatment d 1 (CEF + FM, n = 119). Blood samples were collected 4, 6, and 10 DIM and analyzed for concentrations of haptoglobin and fibrinogen. A group of cows without APM remained untreated and served as controls (n = 9). There were no significant differences between CEF and CEF + FM in the proportion of cows with fever 1 d after the first treatment (33.6 vs. 46.2%), milk yield per milking 10 DIM (7.5 +/- 0.3 vs. 7.6 +/- 0.3 kg in primiparous, 9.6 +/- 0.4 vs. 10.6 +/- 0.4 kg in multiparous cows), prevalence of chronic endometritis 32 to 35 DIM (64.3 vs. 52.2%), and in reproductive performance (31.5 vs. 34.3% conception to first AI, 58.0 vs. 54.6% pregnancy rate, 107.8 +/- 36.9 vs. 101.6 +/- 41.4 d open). Compared with the control, CEF and CEF + FM had significantly greater concentrations of haptoglobin (1.1 +/- 0.28 vs. 1.9 +/- 0.06 and 1.8 +/- 0.07 mg/mL at 4 DIM; 0.3 +/- 0.15 vs. 1.1 +/- 0.06 and 1.2 +/- 0.07 mg/mL at 10 DIM) and fibrinogen (2.2 +/- 0.17 vs. 3.9 +/- 0.14 and 3.7 +/- 0.13 g/L at 4 DIM; 1.9 +/- 0.1 vs. 2.6 +/- 0.1 and 3.0 +/- 0.13 g/L, respectively, at 10 DIM) on all test days. The additional treatment with FM had no effect on these acute phase proteins. In conclusion, the single administration of 2.2 mg/kg of BW of FM in addition to a systemic antibiotic treatment of cows having APM did not result in beneficial effects on clinical cure, milk yield within 6 d after the first treatment, or reproductive performance.
本田间试验的目的是评估对患有急性产后子宫炎（APM）的奶牛，在全身使用抗生素治疗的基础上，单次给予2.2毫克/千克体重（BW）的氟尼辛葡甲胺（FM）的效果。所测试的结果变量包括发热奶牛的比例、产后18至22天以及32至35天泌乳期（DIM）慢性子宫内膜炎的患病率，以及当前泌乳期的繁殖性能指标。此外，还分析了血清中触珠蛋白和纤维蛋白原的浓度。记录首次治疗后6天内的每日产奶量。在产后4至5天通过直肠触诊和阴道镜检查对奶牛进行检查，并测量直肠温度。恶臭的外阴分泌物和体温≥39.5℃是APM的体征。参考组中患有APM的奶牛连续3至5天每天接受1.0毫克/千克体重的头孢噻呋治疗（CEF，n = 119）。在研究组中，奶牛接受与CEF相同的抗生素治疗，并在治疗第1天接受2.2毫克/千克体重的FM治疗（CEF + FM，n = 119）。在产后4、6和10天采集血样，分析触珠蛋白和纤维蛋白原的浓度。一组未患APM的奶牛未接受治疗，作为对照组（n = 9）。在首次治疗后1天发热奶牛的比例（33.6%对46.2%）、产后10天每次挤奶的产奶量（初产奶牛中分别为7.5±0.3对7.6±0.3千克，经产奶牛中分别为9.6±0.4对10.6±0.4千克）、产后32至35天慢性子宫内膜炎的患病率（64.3%对52.2%）以及繁殖性能（首次人工授精的受孕率分别为31.5%对34.3%，妊娠率分别为58.0%对54.6%，空怀天数分别为107.8±36.9对101.6±41.4天）方面，CEF和CEF + FM之间没有显著差异。与对照组相比，在所有测试日，CEF和CEF + FM的触珠蛋白浓度（产后4天分别为1.1±0.28对1.9±0.06和1.8±0.07毫克/毫升；产后10天分别为0.3±0.15对1.1±0.06和1.2±0.07毫克/毫升）和纤维蛋白原浓度（产后4天分别为2.2±0.17对3.9±0.14和3.7±0.13克/升；产后10天分别为1.9±0.1对2.6±0.1和3.0±0.13克/升）显著更高。额外使用FM治疗对这些急性期蛋白没有影响。总之，对患有APM的奶牛在全身使用抗生素治疗的基础上单次给予2.2毫克/千克体重的FM，对临床治愈、首次治疗后6天内的产奶量或繁殖性能没有产生有益影响。
