Suppr
超能文献

[高效液相色谱法测定菠萝叶中的1,3-O-二咖啡酰甘油酯]

[Determination of 1, 3-O-dicaffeoylglycerides from leaves of pineapple by HPLC].

作者信息

Shen Jia, Xie Wei-Dong, Wang Wei, Ding Yi, Du Li-Jun

机构信息

Laboratory of Pharmaceutical Sciences, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 May;32(9):813-5.

PMID:17639981

Abstract

OBJECTIVE

To establish a HPLC method for a new compound 1,3-O-dicaffeoylglycerides determination.

METHOD

The separation was performed in a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) with a mobile phase of 0.1% H3PO4: Acetontrile = 75 : 25 (v/v). The flow rate was 1.0 ml x min(-)1 and the temperature of column was 30 degrees C.

RESULT

A satisfactory separation between 1, 3-O-dicaffeoylglycerides and impurity was obtained. The calibration curve was linear over the concentration range from 0.051 6 microg to 0.516 microg, r = 0.999 9. The average recoveries was 97.1% (RSD 1.3%). The content of 1,3-O-dicaffeoylglycerides in pineapple leaves from three different batches were 0.033%, 0.034% and 0.031% respectively.

CONCLUSION

The method has good selectivity, high recovery and reproducibility, and can be used for the analysis of 1,3-O-dicaffeoylglycerides in pineapple leaves and their quality control.

摘要

目的

建立一种用于测定新化合物1,3 - O - 二咖啡酰甘油酯的高效液相色谱法。

方法

采用Kromasil C18柱（4.6 mm×150 mm，5μm）进行分离，流动相为0.1% H3PO4：乙腈 = 75 : 25（v/v）。流速为1.0 ml·min⁻¹，柱温为30℃。

结果

1,3 - O - 二咖啡酰甘油酯与杂质之间实现了良好的分离。校准曲线在0.051 6μg至0.516μg浓度范围内呈线性，r = 0.999 9。平均回收率为97.1%（相对标准偏差1.3%）。来自三个不同批次菠萝叶中1,3 - O - 二咖啡酰甘油酯的含量分别为0.033%、0.034%和0.031%。