Kimura Tohru, Koike Takako, Matsunaga Tomoko, Sazi Toshiyuki, Hiroe Takeshi, Kubota Mitsuko
Center for Experimental Animals, National Institute of Natural Sciences, Okazaki, Japan.
J Am Assoc Lab Anim Sci. 2007 Jul;46(4):33-8.
We clinically and clinicopathologically investigated the immobilizing and sedative effects of a medetomidine-midazolam (MM) combination in Japanese monkeys (Macaca fuscata) and its antagonism with atipamezole. MM (medetomidine, 60 microg/kg; midazolam, 0.3 mg/kg) was administered intramuscularly to each monkey (n = 11). All animals were laterally recumbent within 13 +/-6 min after administration of MM. This combination induced deep sedation accompanied by analgesia, muscle relaxation, and markedly depressed arousal reactions to external stimuli. After administration of atipamezole (240 microg/kg intramuscularly), the animals recovered rapidly and smoothly to their normal postures within 10 +/-2 min. In this study, the hematologic and serum biochemical parameters of Japanese monkeys given MM did not differ significantly from those of Japanese monkeys under general anesthesia via ketamine. Salivary a-amylase activities (stress indexes) ranged from 4 to 99 kU/l in Japanese monkeys, similar to levels measured in humans. An important advantage of MM was that its effects were reversible with atipamezole. We have confirmed that MM is valuable as a chemical restraint agent in Japanese monkeys for various experimental procedures.
我们对美托咪定 - 咪达唑仑（MM）组合在日本猕猴（食蟹猴）中的制动和镇静作用及其与阿替美唑的拮抗作用进行了临床和临床病理研究。将MM（美托咪定，60微克/千克；咪达唑仑，0.3毫克/千克）肌肉注射给每只猴子（n = 11）。给药后，所有动物在13±6分钟内呈侧卧位。这种组合诱导了深度镇静，伴有镇痛、肌肉松弛，并显著降低了对外界刺激的觉醒反应。肌肉注射阿替美唑（240微克/千克）后，动物在10±2分钟内迅速平稳地恢复到正常姿势。在本研究中，给予MM的日本猕猴的血液学和血清生化参数与通过氯胺酮进行全身麻醉的日本猕猴相比，差异不显著。日本猕猴唾液α - 淀粉酶活性（应激指标）范围为4至99千单位/升，与人类测量水平相似。MM的一个重要优点是其作用可被阿替美唑逆转。我们已经证实，MM作为一种化学制动剂，在日本猕猴进行各种实验程序时具有重要价值。
